肿瘤坏死因子相关凋亡诱导配体对卵巢癌裸鼠移植瘤SKOV3细胞的促凋亡研究  

Research on the Impact of TRAIL on Apoptosis of SKOV3 Cell Line Ovarian Tumor Xenografts in Nude Mice

作　　者：李春梅[1] 彭芝兰[1] 杨开选[2] 刘英[2] 黄琴[2] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041 [2]四川大学华西第二医院病理科,成都610041

出　　处：《华西医学》2011年第4期481-484,共4页West China Medical Journal

基　　金：国家自然科学基金面上资助项目(30973192)~~

摘　　要：目的研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)蛋白对SKOV3移植瘤细胞半胱天冬氨酸蛋白酶-3(Caspase-3)表达的影响及其与肿瘤细胞凋亡的关系。方法建立雌性裸小鼠SKOV3移植瘤24只,随机分为4组,每组6只。TRAIL组单用重组人TRAIL蛋白(10μg/kg),顺铂(DDP)组单用DDP(3 mg/kg),TRAIL+DDP组联合使用TRAIL蛋白(10μg/kg)和DDP(3 mg/kg),空白对照组给予0.5 mL生理盐水。经处理后,各组的组织切片用免疫组织化学染色检测Caspase-3的表达和末端脱氧核苷酸转移酶介导核苷酸缺口标记技术(TUNEL)检测肿瘤细胞凋亡指数。结果 Caspase-3的表达水平在TRAIL组(171.67±14.38)、DDP组(172.50±14.75)、联合组(230.00±40.99)中均明显高于对照组(135.83±16.25)(P<0.05)。SKOV3移植瘤细胞凋亡指数在空白对照组、TRAIL组、DDP组和联合组分别为16.67±5.43、33.17±8.42、24.33±4.59和40.50±6.16,TRAIL组和联合组细胞凋亡指数较空白对照组和DDP组明显增高(P<0.05)。结论 TRAIL蛋白使卵巢癌移植瘤细胞的Caspase-3表达增强,TRAIL蛋白促进肿瘤细胞凋亡发生。Objective To investigate the effects of Tumor necrosis factor-related apoptosis-inducing ligand（TRAIL） on the expression of Cysteine/aspartic acid specific protease-3（Caspase-3） in SKOV3 ovarian tumor cells and its relationship with the apoptosis of the ovarian tumor xenografts in nude mice.Methods Twenty-four nude mice with SKOV3 cell line ovarian tumor were randomly divided into four groups with 6 in each group.TRAIL（10 μg/kg） was given to the mice in the TRAIL group;DDP（3 μg/kg） was given to the mice in the DDP group;TRAIL（10 μg/kg） and DDP（3 μg/kg） were given to the mice in the TRAIL＋DDP group;and 0.5 mL of saline solution was give to the mice in the control group.The expression of Caspase-3 was detected with immunohistochemistry.The apoptosis index（AI） of cells was determined by Terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling（TUNEL）.Results The expression of Caspase-3 in the TRAIL group（171.67±14.38）,DDP group（172.50±14.75）,and TRAIL＋DDP group（230.00±40.99） was significantly higher than that in the control group（135.83±16.25）（P0.05）.The apoptosis index for the control group,TRAIL group,DDP group and TRAIL＋DDP group was 16.67±5.43,33.17±8.42,24.33±4.59,and 40.50±6.16,respectively.The apoptosis index for the TRAIL group and the TRAIL＋DDP group was significantly higher than that in the control group and the DDP group（P0.05）.Conclusion Soluble TRAIL has an effect on enhancing the expression of Caspase-3 in implanted tumor in nude mice.TRAIL protein can inhibit the growth of SKOV3 cells in nude mice by inducing cell apoptosis.

关 键 词：卵巢癌 肿瘤坏死因子相关凋亡诱导配体 末端脱氧核苷酸转移酶介导核苷酸缺口标记技术 半胱天冬氨酸蛋白酶-3 凋亡 小鼠 

分 类 号：R737.31[医药卫生—肿瘤]