3株弥漫大B细胞淋巴瘤细胞系的鉴定与临床病理相关分析  

Identification of three diffuse large B-cell lymphoma cell lines and clinicopathological correlation analysis

作　　者：王志强[1] 吴自勍[2] 黎相照[1] 杨建芳[1] 黄学平[1] 李锋[1] 周新华[1] 赵彤[1,2] 

机构地区：[1]南方医科大学附属南方医院病理科,广东广州510515 [2]南方医科大学广东省分子肿瘤病理重点实验室,广东广州510515

出　　处：《热带医学杂志》2011年第4期361-364,F0002,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金(30770908;81071941)

摘　　要：目的鉴定OCI-Ly1、OCI-Ly8、OCI-Ly103株弥漫大B细胞(diffuselargeB-celllymphoma,DLBCL),并进行分子亚类分型及临床病理相关分析。方法采用细胞块切片HE染色观察细胞形态,运用CD3、CD20、CD10、Bcl-6、MUM-1抗体进行免疫标记确定其分子亚类;MTT法测定细胞的增殖活力。结果 OCI-Ly1和OCI-Ly8细胞形态以中心母细胞为主,OCI-Ly10以免疫母细胞为主。3株细胞CD20和CD10均弥漫阳性,CD3阴性。OCI-Ly1个别细胞Bcl-6阳性,MUM-1阴性;OCI-Ly8细胞Bcl-6、MUM-1均阴性;OCI-Ly10个别细胞Bcl-6阳性,MUM-1散在或簇状阳性,CD10和MUM-1的表达具有互补现象,在36例DLBCL临床病理标本中发现有7例(19%)CD10和MUM-1表达互补。OCI-Ly10增殖能力显著高于OCI-Ly1、OCI-Ly8(P<0.001)。结论 OCI-Ly1和OCI-Ly8属于GCB型,OCI-Ly10属于NC型(non-classfied);OCI-Ly10细胞系及组织标本CD10和MUM-1的表达互补现象有待进一步研究。Objective To identify the molecular subgroup classification and carry out clinical pathological correlation analysis of three DLBCL cell lines（OCI-Ly1,OCI-Ly8,OCI-Ly10）.Methods The morphological characteristics of the cell lines were observed using hematoxylin eosin staining on cell blocks.Subgroup of cell lines was determined by the immunohistochemistry staining on cell blocks using the monoclonal antibodies（CD3,CD20,CD10,Bcl-6,MUM-1）.Cell proliferation activity was detected by MTT.Results OCI-Ly1 and OCI-Ly8 were centroblastic variants.OCI-Ly10 was immunoblastic variant.Three cell lines（OCI-Ly1,OCI-Ly8,OCI-Ly10） were diffusely positive for CD20 and CD10,and negative for CD3.OCI-Ly1 showed low frequency of Bcl-6＋ cells,and was negative for MUM-1.OCI-Ly8 was negative for Bcl-6 and MUM-1.OCI-Ly10 also showed low frequency of Bcl-6＋ cells,and was clustered positive for MUM-1.Complementary expression of CD10 and MUM-1 were detected by the immunohistochemistry staining on OCI-Ly10 and 7 out of 36 DLBCL clinical samples（19%）.Cell proliferation activity of OCI-Ly10 was significantly higher than that of OCI-Ly1 and OCI-Ly8（P 0.001）.Conclusions OCI-Ly1 and OCI-Ly8 are GCB type and OCI-Ly10 is NC type（non-classified）.A further investigation of complementary expression of CD10 and MUM-1 in clinical samples is fairly necessary in the future.

关 键 词：细胞系 细胞周期 细胞增殖 免疫表型 

分 类 号：R363.2[医药卫生—病理学]