大鼠骨骼肌卫星细胞的原代培养和鉴定  

Cultivation and identification of rat skeletal muscle satellite cells

作　　者：陈思凡[1,2] 李文学[1] 陈建玲[1,2] 杨光宇[1] 刘华章[1] 朱伟[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510080 [2]中山大学公共卫生学院,广东广州510080

出　　处：《热带医学杂志》2011年第4期395-397,F0003,共4页Journal of Tropical Medicine

基　　金：广州市科技计划项目(2009Z1-E061)

摘　　要：目的采用组织块培养技术探索大鼠骨骼肌卫星细胞的原代培养方法。方法以成年SPF级Sprague-Dawley大鼠为研究对象,采用组织块培养法获取大鼠骨骼肌卫星细胞,并与C2C12成肌细胞进行比较,对两种细胞进行形态学研究及采用免疫荧光和免疫组织化学法测定两种细胞α-actin蛋白和Desmin蛋白的表达及分布,从而对骨骼肌卫星细胞进行鉴定。结果通过组织块培养法获取的细胞增殖旺盛,分化良好。免疫细胞荧光和免疫组织化学实验结果显示,α-actin蛋白和Desmin蛋白在两种细胞胞浆中均有分布。结论用组织块培养法获取的骨骼肌卫星细胞具有良好的增殖与分化能力,用此种方法可培养出高纯度的骨骼肌卫星细胞。Objective To explore the primary culture method of skeletal muscle satellite cells.Methods Skeletal muscle satellite cells were obtained from the specific pathogen free Sprague-Dawley rats,and C2C12 myoblast cell line was used as control.The cellular morphology was observed and photographed under an inverted microscope.The expression of α-actin and desmin was assayed with immunofluorescence and immunohistochemical staining method,respectively..Results The morphological feature of primary cultured skeletal muscle satellite cells was similar with the myoblast C2C12 cell line.Immunofluorescence and immunohistochemistry staining showed that the pattern of α-actin and desmin protein staining in the cytoplasm of skeletal muscle satellite cells was similar to C2C12.Conclusion Skeletal muscle satellite cells can be obtained using the tissue culture method.

关 键 词：骨骼肌卫星细胞 组织块培养技术 Desmin蛋白 

分 类 号：R394.2[医药卫生—医学遗传学]