利用SSR、EST．SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析  

Analysis of feed conversion ratio,bodyweight,body thickness traits of common carp (Cyprinus carpio L.) using SSR,EST-SSR,SNP markers

作　　者：王宣朋[1,2] 张晓峰[1] 李文升[1,2] 张天奇[1,3] 孙效文[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]上海海洋大学水产与生命学院,上海201306 [3]大连水产学院生命科学与技术学院,辽宁大连116023

出　　处：《中国水产科学》2011年第3期565-573,共9页Journal of Fishery Sciences of China

基　　金：国家973计划项目(2010CB126305);农业部公益性行业科研专项(200903045)

摘　　要：利用Windows Map Manager2.0的标记回归法对鲤(Cyprinus carpio L.)F2群体进行单标记定位分析。用91个SSR标记、33个EST标记、364个SNP标记对鲤进行全基因组扫描,结果得到与食物转化率、体厚、体质量3个性状显著相关(P<0.05)的标记,分别为27、35、27个,其中与食物转化率相关的标记SNP0041、SNP0044,其相关性达到极显著水平(P<0.001),贡献率分别达到15%、16%。这些标记可作为分子标记辅助育种的参考标记。将得到的与食物转化率显著相关的EST座位HLJE14、HLJE253和与体质量显著相关的HLJE253座位在NCBI上进行BLAST比对,结果显示HLJE253与斑马鱼上编码ORF2结构蛋白的基因相关,相关程度达61%。将得到的与3种性状相关的SNP标记在NCBI上进行序列查找,通过Blast比对找到相关的基因,并对可能的功能作了注释。An F2 hybrid common carp gynogenetic line including 68 individuals was used as mapping panel. Markers were used to make marker regression analysis based on pseudo-testcross strategy using Windows Map Manager2.0. Ninety-one SSR markers and 33 EST makers and 364 SNP markers were selected to analyze the genomic DNA of 68 progenies derived from common carp full-sib family. Twenty-seven loci were discovered that had significant impact on feed conversion efficiency and thirty-five loci were significantly related with body thickness （P〈0.05）, while twenty seven loci had significant impact on body weight （P〈0.05）. SNP0041 and SNP0044 were related with feed conversion ratio significantly （P〈0.01）, contributing to 15% and 16%, respec- tively. These markers can be used as molecular marker assisting breeding. Markers were used to screen the protein and nucleotide database in the National Center for Biotechnology Information （NCBI） in order to find the homologies. Blasting results showed that HLJ253 is highly correlated （61%） with the gene encoding structural protein （ORF2） ofzebrafish. SNP makers associated with three traits were sequenced on NCBI in order to find related genes and possible functions.

关 键 词：鲤 EST-SSR SNP 食物转化率 体厚 体质量 

分 类 号：S917[农业科学—水产科学]