IL-12诱导类风湿关节炎和骨关节炎外周血T淋巴细胞STAT4活化状态的比较  

Comparison of the status of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis and osteoarthritis

作　　者：孙振杰[1] 张育军 孙铁铮[3] 熊奡[4] 刘瑞波[1] 吕厚山[3] 

机构地区：[1]北京市第六医院骨科,北京100007 [2]北京大学人民医院中心实验室 [3]北京大学人民医院关节病诊疗研究中心,北京100035 [4]深圳市北京大学深圳医院骨科

出　　处：《中国骨伤》2011年第4期295-298,共4页China Journal of Orthopaedics and Traumatology

基　　金：首都医学发展基金资助课题(编号:TS199923)~~

摘　　要：目的:比较类风湿关节炎和骨关节炎外周血T淋巴细胞在IL12作用下诱导STAT4酪氨酸磷酸化水平的差异。方法:2007年5月至2009年8月分离提纯20例类风湿关节炎患者[均为女性;年龄28～55岁,平均(45.0±13.0)岁]和20例骨关节炎患者[均为女性;年龄55～75岁,平均(67.0±9.6)岁]的外周血T淋巴细胞,流式细胞仪进行T淋巴细胞纯度鉴定。用50ng/mlIL12分别作用0、10、30、60min后,提取蛋白进行浓度测量,然后进行WesternBlot检测,经蛋白条带灰度扫描,比较IL12作用各时间点类风湿关节炎和骨关节炎T淋巴细胞STAT4酪氨酸磷酸化水平的差异。结果:经过对外周血单核细胞的分离纯化,T细胞纯度达到91%以上;IL12在对骨关节炎和类风湿关节炎T细胞作用30min后达到活化峰值,在各个时间点(10、30、60min)类风湿关节炎外周血T淋巴细胞活化水平均高于骨关节炎。结论:类风湿关节炎外周血T淋巴细胞在IL12作用下STAT4异常活化。Objective：To compare the levels of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis（RA） and osteoarthritis（OA）.Methods：From May 2007 to August 2009,peripheral blood mononuclear cells（PBMCs） were isolated from RA patients[RA group,all the cases were female,the age was from 28 to 55 years with an average of（45.0±13.0） years] and OA patients[OA group,all the cases also were female;the age was from 55 to 75 years with an average of（67.0±9.6） years].The purity of T-lymphocytes from PBMCs was accredited by flow cytometry.The IL-12 of 50 ng/ml added in T-lymphocytes,the levels of STAT4 tyrosine phosphorylation were detected by western blot after different time intervals（0,10,30,60 min）.Results：The purity of T-lymphocytes were above 91% through diremption and depuration for peripheral blood monouclear cells.The levels of STAT4 tyrosine phosphorylation in T-lymphocytes from RA induced by IL-12 were higher than that from OA in the different times（10,30,60 min）;after 30 min,its levels from RA and OA achieved to crest value.Conclusion：STAT4 in peripheral T-lymphocytes of rheumatoid arthritis was more easily to be activated than osteoarthritis.

关 键 词：关节炎 类风湿 淋巴细胞 白细胞介素-12 

分 类 号：R734.2[医药卫生—肿瘤]