SKI-Ⅱ抑制Sphk1的表达对胃癌细胞株SGC7901细胞周期的影响  

Effect of SKI-Ⅱ on SGC7901 cell cycle by inhibiting the Sphk1 expression

作　　者：朱祖安[1] 费素娟[1] 刘磊[2] 张秋月[1] 孙旻[1] 刘莹[2] 

机构地区：[1]徐州医学院附属医院消化内科,江苏徐州221000 [2]徐州医学院病理学教研室,江苏徐州221000

出　　处：《山东大学学报（医学版）》2011年第4期70-74,共5页Journal of Shandong University:Health Sciences

基　　金：江苏省教育厅自然科学基金资助项目(05KJD320234);徐州医学院肿瘤生物治疗重点实验室开放课题资助项目(C0806)

摘　　要：目的研究SKI-Ⅱ对胃癌SGC7901细胞周期的阻滞作用,并探讨其相关分子机制。方法常规培养胃癌SGC7901细胞,SKI-Ⅱ单用或联合顺铂干预,流式细胞术检测细胞周期;免疫细胞化学、Western-blot检测药物作用后细胞中Sphk1、P27的表达。结果 SKI-Ⅱ单用或联合顺铂干预48 h后,各用药组细胞周期停滞在G0/G1期比例增多,与阴性对照组相比差异有统计学意义(P<0.05);联合用药组比SKI-Ⅱ单用组作用明显(P<0.05);SGC7901细胞中P27阳性表达率增加,Sphk1阳性表达率减少,与阴性对照组相比差异有统计学意义(P<0.05)。Pearson相关分析显示:SGC7901细胞中Sphk1与P27的表达呈负相关。结论 SKI-Ⅱ通过抑制Sphk1的表达进而上调P27的表达诱导肿瘤细胞周期阻滞,抑制肿瘤细胞增殖。Objective To study the effect of SKI-Ⅱ on arresting gastric cancer SGC7901 cell cycle,and explore the mechanisms.Methods SGC7901 cells were treated with SKI-Ⅱ alone or in combination with cisplatin,and the cell cycle was analyzed by flow cytometry.Expressions of Sphk1 and P27 were detected by immunocytochemistry and Western-blot.Results After being treated with SKI-Ⅱ alone or in combination with cisplatin for 48h,the ratio of cells arresting in the G0/G1 phase was increased,compared with the control group（P0.05）;the effect of combined treatment group was more significant（P0.05）.Expression of P27 in SGC7901 cells was increased,while expression of Sphk1 was decreased,compared with the control group（P0.05）.Pearson correlation analysis showed that expressions of Sphk1 and P27 were negatively related.Conclusion SKI-Ⅱ could arrest gastric cancer SGC7901 cell cycle and inhibit cell growth through increased expression of P27,which was regulated by inhibiting expression of Sphk1.

关 键 词：SKI-Ⅱ SGC7901细胞 胃肿瘤 细胞周期 

分 类 号：R735.2[医药卫生—肿瘤]