赖氨酰氧化酶在乳腺癌中的表达及临床意义  

Expression of lysyl oxidase in breast cancer and its clinical significance

作　　者：杨华伟[1] 刘剑仑[1] 蒋奕[1] 唐玮[1] 韦薇[1] 黄真[1] 

机构地区：[1]广西医科大学附属肿瘤医院乳腺疾病中心外科,南宁530021

出　　处：《山东大学学报（医学版）》2011年第4期136-140,共5页Journal of Shandong University:Health Sciences

基　　金：广西科学研究与技术开发计划项目基金资助(桂科攻0816004-14)

摘　　要：目的研究赖氨酰氧化酶(LOX)在乳腺癌组织、正常乳腺组织及乳腺良性病变组织中的表达,并与乳腺癌常规预后指标比较,探讨其与乳腺癌侵袭/转移的关系。方法逆转录聚合酶链式反应(RT-PCR)检测56例乳腺癌组织、56例正常乳腺组织及20例乳腺良性病变组织中LOX mRNA与低氧诱导因子1(HIF-1α)mRNA的表达;应用免疫组织化学技术检测相应组织中LOX蛋白及HIF-1α的表达,并行统计学分析。结果 LOX mRNA在乳腺癌组织、正常乳腺组织及乳腺良性病变组织中的表达率分别为57.14%、32.14%、25.00%,乳腺癌组织中LOXmRNA的表达率明显高于正常乳腺组织及乳腺良性病变组织(P<0.01);LOX蛋白在乳腺癌组织、正常乳腺组织及乳腺良性病变组织中的表达率分别为48.21%、26.78%、20.00%,乳腺癌组织中LOX蛋白的表达率明显高于正常乳腺组织及乳腺良性病变组织(P<0.05);LOX mRNA及LOX蛋白在不同临床分期及淋巴结转移中的表达率差异有统计学意义(P<0.05),其中Ⅲ、Ⅳ期乳腺癌LOX mRNA及LOX蛋白的表达率明显高于Ⅰ、Ⅱ期(P均<0.01),但在不同年龄及肿瘤大小患者的癌组织中LOX mRNA及LOX蛋白的表达率差异无统计学意义(P>0.05);LOX蛋白的表达与HIF-1α呈正相关(r=0.368)。结论 LOX蛋白及LOX mRNA在乳腺癌组织中的表达率高于正常乳腺组织及乳腺良性病变组织,LOX与乳腺癌的发生可能有关;LOX高表达的乳腺癌其恶性程度更高,更易发生远处转移;HIF-1α与LOX在乳腺癌的侵袭/转移过程中具有协同作用。Objective To study expression of lysyl oxidase（LOX） in human breast cancer and explore its relationship with clinical prognostic features（patient′s age,tumor size,lymph node metastasis,clinical stage,ER,PR and HER-2 state） and breast cancer invasion / metastasis.Methods mRNA expressions of LOX and HIF-1α in tissues were detected in 56 cases of human breast cancer,56 cases of normal breast and 20 cases of breast benign lesion by reverse transcriptase polymerase chain reaction（RT-PCR）,and protein expressions of LOX and HIF-1α by S-P immunohistochemistry.Results Expression rates of LOX mRNA in breast cancer,normal breast tissues and breast benign lesion tissues were 57.14%,32.14% and 25.00%,respectively,and statistical differences were significant among them（P0.01）.Expression rates of the LOX protein in breast cancer,normal breast tissues and breast benign lesion tissues were 48.21%,26.78% and 20.00%,respectively,and statistical differences were significant among them（P0.05）.Expressions of LOX mRNA and protein were associated with the clinical stage and lymph node metastasis（P0.05）,and were significantly higher in stage Ⅲ and Ⅳ breast cancer than those in stageⅠ and Ⅱ（P0.01）,while they had no correlation with age and tumor size（P0.05）.The LOX protein had significant positive correlation with HIF-1α protein expression in breast cancer（r=0.368）.Conclusion LOX protein and mRNA expressions are higher in breast cancer compared with normal breast tissues and breast benign lesion tissues,and LOX is probably relevant to occurrence of breast cancer.Higher expression of LOX can increase the malignant degree of human breast cancer cells and facilitates metastasis.LOX and HIF-1α have a synergistic effect in breast cancer invasion and metastasis.

关 键 词：乳腺肿瘤 赖氨酰氧化酶 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]