Toxicity of the brominated flame retardant tris-(2,3-dibromopropyl) isocyanurate in zebrafish (Danio rerio)  

作　　者：ZHANG Xu LI Juan CHEN MinJie WU Le ZHANG Chi ZHANG Jie ZHOU QunFang LIANG Yong 

机构地区：[1]Key Laboratory of Subtropical Agriculture and Environment, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China [2]School of Medicine, Jianghan University, Wuhan 430056, China [3]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beifing 100085, China

出　　处：《Chinese Science Bulletin》2011年第15期1548-1555,共8页

基　　金：supported by the National Natural Science Foundation of China (20890112 and 20907017);the National Basic Research Program of China (2009CB421605);the National High Technology R&D Program of China (2007BAC27B01);State Key Laboratory of Environmental Chemistry and Ecotoxicology (KF2009-05)

摘　　要：Tris-(2,3-dibromopropyl) isocyanurate (TBC) is a heterocyclic brominated flame retardant that was recently detected in the environment in China.TBC is semi-volatile and can accumulate in the lipid of some species,but little is known about its effect on aquatic organisms.We exposed adult zebrafish to 0,0.25,1 and 4mg/L TBC for 28 d and measured the effect on survival,growth,histopathology,hormone levels,enzyme activity,and gene expression.TBC exposure had no effect on survival or growth.We observed significant damage to the liver and gill,including hepatocellular swelling and fatty degeneration in the liver as well as proliferation and edema of epithelial cells in the gills.In addition,exposure to 4mg/L TBC induced proliferation of goblet cells in the intestine of both sexes,acellular areas in the testis,and thinly scattered vitellogenic granules in vitellogenic oocytes.TBC exposure had no effect on the levels of thyroid hormones,testosterone,estradiol,liver superoxide dismutase activity,malondialdehyde content,and brain cholinesterase activity.By contrast,hepatic vitellogenin and cytochrome P4501A gene expression was significantly down-regulated in both male and female zebrafish in response to TBC exposure.Our results suggest that exposure to TBC causes a variety of potential reproductive and endocrine toxic effects.Tris-（2,3-dibromopropyl） isocyanurate （TBC） is a heterocyclic brominated flame retardant that was recently detected in the environment in China. TBC is semi-volatile and can accumulate in the lipid of some species, but little is known about its effect on aquatic organisms. We exposed adult zebrafish to 0, 0.25, 1 and 4 mg/L TBC for 28 d and measured the effect on survival, growth, histopathology, hormone levels, enzyme activity, and gene expression. TBC exposure had no effect on survival or growth. We observed significant damage to the liver and gill, including hepatocellular swelling and fatty degeneration in the liver as well as proliferation and edema of epithelial cells in the gills. In addition, exposure to 4 mg/L TBC induced proliferation of goblet cells in the intestine of both sexes, acellular areas in the testis, and thinly scattered vitellogenic granules in vitellogenic oocytes. TBC exposure had no effect on the levels of thyroid hormones, testosterone, estradiol, liver superoxide dismutase activity, malondialdehyde content, and brain cholinesterase activity. By contrast, hepatic vitellogenin and cytochrome P4501A gene expression was significantly down-regulated in both male and female zebrafish in response to TBC exposure. Our results suggest that exposure to TBC causes a variety of potential reproductive and endocrine toxic effects.

关 键 词：溴化阻燃剂 异氰脲酸酯 毒性作用 斑马鱼 超氧化物歧化酶活性 丙基 二溴 基因表达 

分 类 号：X174[环境科学与工程—环境科学]