同种异体结膜移植红细胞免疫功能的实验研究  

An experimental study on red blood cell immune in allogenic conjunctival transplantation

作　　者：杨敬昌[1] 于秀敏[2] 李守明[1] 马义宾[1] 李新华[3] 邓文[3] 

机构地区：[1]泰安市中心医院眼科,271000 [2]山东省立医院眼科 [3]泰山医学院免疫学教研室

出　　处：《中国实用眼科杂志》1999年第9期523-526,共4页Chinese Journal of Practical Ophthalmology

摘　　要：目的： 研究同种异体结膜移植术后红细胞的免疫学机理， 进一步丰富结膜移植排斥反应理论。方法： 对 １２ 只青紫兰兔进行交互同种异体结膜移植， 于术前、术后１、２、３、４、５、６ 周检测外周血红细胞 Ｃ３ ｂ 受体花环率 （ Ｒ Ｂ Ｃ— Ｃ３ ｂ Ｒ Ｒ）， 红细胞免疫复合物花环率 （ Ｒ Ｂ Ｃ— Ｉ Ｃ Ｒ）， 红细胞粘附肿瘤细胞花环率 （ Ｒ Ｂ Ｃ— Ｔ Ｃ Ｒ）， 对所得数据， 以术前数据组为对照组， 进行均数的ｔ 检验。结果： Ｒ Ｂ Ｃ— Ｃ３ ｂ Ｒ Ｒ于术后第二周开始升高， 明显高于对照组 （ Ｐ＜ ００５）， 到第三周达最高峰 （ Ｐ＜ ０００１）， 然后缓慢降低， 持续到第五周 （ Ｐ＜ ００５）。 Ｒ Ｂ Ｃ— Ｉ Ｃ Ｒ于术后第三周开始降低 （ Ｐ＜ ００５）， 持续到第四周 （ Ｐ＜ ０００１）， 第五周始检测结果与对照组比较无显著性差异 （ Ｐ＞ ００５）。 Ｒ Ｂ Ｃ— Ｔ Ｃ Ｒ 于术后第一周开始升高 （ Ｐ＜ ００１）， 第三周达高峰 （ Ｐ＜ ００５）， 持续到第四周 （ Ｐ＜００５）。结论： 在移植早期 （术后 ４ 周内）， 红细胞免疫功能增强， 有促排斥作用。红细胞对移植术后机体产生的大量免疫复合物的清除起重要作用， 移植术第四周后，Objective:To study the mechanism of red blood cell immunology after allogenic conjunctival transplantation,enrich the theory of conjunctival graft rejection and offer the theoretical basis of the allogenic conjuntival transplantation in human.Methods:12 pigmented rabbits received allogenic conjunctival transplantation.The rate of red blood cell C 3b receptor rosette (RBC—C 3bRR),immune complex rosette (RBC—ICR)and tumor cell rosette (RBC—TCR)were measured respectively on the day before operation and 1,2,3,4,5,6 week after operation,the means of each data group were test by t test,the data group of pre operation was thought to be control group.Results:RBC—C 3bRR rised in 2nd week (P<0 05)and rather high in 3rd week (P<0 001),then decreased slowly during 4th and 5th week,but still higher than the control group (P<0 05)until 6th week of post operation.RBC—ICR decreased during 3rd and 4th week (P<0 05)of post operation,and the differences had no significance (P>0 05)after 4th week.RBC—TCR rised in 1st week (P<0 01)and gained the peak value in 3rd week (P<0 05),then kept high in 4th week (P<0 05)after the operation.The differences of the means had no significance after 4th week (P>0 05).Conclusions:Red blood cell partakes the graft rejection process of allogenic conjunctival transplantation,the immune function of RBC gets stronger than the normal,and it also can promote the rejection during the early time of post operation(before 4th week after operation).RBC plays an important role in the transport of circulated immune complex(CIC).There is an immunotolerance showes in 4th week later after conjuctival transplantation,RBC immune function level also decreases in the mean time.

关 键 词：结膜移植 同种异体移植 红细胞免疫功能 

分 类 号：R777.3[医药卫生—眼科] R779.6[医药卫生—临床医学]