miR-193b对胃癌细胞浸润和转移的影响  

The effect of miR-193b on the metastatic and invasive capacity of gastric cancer cells

作　　者：周海燕[1] 王宽松[1] 胡忠良[1] 文继舫[1] 

机构地区：[1]中南大学基础医学院病理学系,湖南长沙410013

出　　处：《中国普通外科杂志》2011年第4期377-382,共6页China Journal of General Surgery

摘　　要：目的探讨miR-193b转染对胃癌细胞浸润、转移的影响及其作用机制。方法应用microRNA芯片检测TGF-β1处理胃癌细胞BGC823前后miRNA的差异表达谱;应用划痕实验,transwell迁移浸润及裸鼠成瘤等实验分别检测瞬时转染miR-193b抑制剂前后胃癌细胞株生物学行为的变化。结果 TGF-β1处理胃癌细胞BGC823前后miRNA的表达谱存在6个差异表达,包括3个(miR-27a,miR-29b-1和miR-194)表达上调,3个(miR-193b,miR-574-3p和miR-130b)表达下调。转染miR-193b抑制剂后,胃癌细胞株BGC823迁移、浸润和转移的能力明显增强。结论 TGF-β1可能通过下调miR-193b的表达负向调控胃癌细胞的迁移、浸润和转移。Objective To study the effect of miR-193b on metastasis and invasion of gastric carcinoma cell line BGC823 and its mechanism.Methods The difference of microRNA expression of gastric cancer cell BGC823 before and after the cells treated by TGF-β1 was detected by miRNA array,and the changes of biological behavior of gastric cancer BGC823 cell line after transfection with miR-193b inhibitor was measured by migration,invasion and metastasis test.Results There was 6 differential microRNA profile expression after TGF-β1 treatment of BGC823.Transfection of miR-193b inhibitor enhanced the ability of migration,invasion and metastasis of gastric carcinoma cell line BGC823.Conclusions TGF-β1 can enhance the ability of migration,invasion and metastasis of gastric carcinoma cell line BGC823 through decreasing the expression of miR-193b.

关 键 词：胃肿瘤/病理学 microRNA芯片 miR-193b UPA 肿瘤转移 肿瘤浸润 迁移 

分 类 号：R730.2[医药卫生—肿瘤]