新疆维吾尔族儿童乳牙龋与变形链球菌基因型相关性的初步研究  

Preliminary study on genotypic diversity of Streptococcus mutans in Xinjiang Uygur preschool children

作　　者：林静[1] 赵今[1] 努尔比亚木·麦麦提依明[1] 李新尚[1] 

机构地区：[1]新疆医科大学第一附属医院牙体牙髓科,乌鲁木齐 830054

出　　处：《中华口腔医学杂志》2011年第5期267-271,共5页Chinese Journal of Stomatology

摘　　要：目的探讨新疆维吾尔族不同龋敏感儿童口腔中变形链球菌基因多态性与乳牙龋发生的关系，以期为新疆维吾尔族儿童乳牙龋危险性预测和防治提供依据。方法选取新疆乌鲁木齐市3-5岁维吾尔族高龋（龋失补牙数≥5）和无龋（龋失补牙数=0）儿童各17名为研究对象，收集唾液样本，轻唾-杆菌肽琼脂培养基分离培养变形链球菌，典型菌落行革兰染色、生化鉴定并细菌保种，提取基因组DNA，采用OPA02、OPA05和OPAl3三个随机引物对变形链球菌临床分离株行随机引物聚合酶链反应基因分型及聚类分析。结果分别从高龋和无龋儿童口腔中分离鉴定变形链球菌81和62株，共得N40种不同的基因型。17例高龋儿童中9例携带1种基因型，5例携带2种基因型，3例携带3种基因型；17名无龋儿童中14名携带1种基因型，3名携带2种以上基因型。Spearman相关结果显示基因多态性与龋敏感相关（r=0．342，P〈0．05）。结论维吾尔族儿童口腔中变形链球菌菌株间存在明显的基因多态性，高龋儿童口腔中定植的变形链球菌有多种基因型，个体携带基因型的种类数与其致龋能力相关。Objective To investigate the relationship between genotypic diversity of Streptococcus mutans（Sm） and deciduous teeth caries in Xinjiang Uygur autonomous region. Methods Isolates of Sm were obtained from 17 caries-free and 17 caries-active Uygur preschool children aged from 3 to 5 years. A total of 143 isolates were subcuhured, biochemically characterised and identified by polymerase chain reaction（PCR） as Sm and Streptococcus sobrinus （Ss）. Then the Sm isolates were genotyped by arbitrarily primed-PCR（AP-PCR）. Results A total of 81 Sm isolates from caries-active subjects and 62 isolates from caries-free subjects were identified by PCR and forty distinct genotypes identified from 143 clinicalisolates. In seventeen caries-active group, 9 children had only 1 genotype, 5 children had 2 genotypes, 3 children had 3 genotypes. In seventeen caries-free group, 14 children had only 1 genotype, 3 children had more than 2 genotypes. The Spearman correlation test showed a strong association between genotypic diversity and caries experience （ r = 0. 342, P 〈 0. 05 ）. Conclusions Isolates of Sm in caries-active Uygur preschool children show apparent more genetic diversity than those in caries-free children. The genotypes of isolates might be related to differences in caries susceptibility.

关 键 词：维吾尔族 链球菌 变异 基因型 随机引物聚合酶链反应 

分 类 号：R788.1[医药卫生—口腔医学]