慢性牙周炎孕鼠胎盘中肿瘤坏死因子的表达  

Expression of tumor necrosis factor in placenta tissue of pregnant rats with chronic peridontitis

作　　者：刘景[1] 钟良军[1,2] 史芳川[2] 张蕾[1] 张琼[1] 孙大磊[1] 

机构地区：[1]新疆医科大学第一附属医院牙周黏膜科,乌鲁木齐 830054 [2]杭州师范大学临床医学院附属医院口腔科

出　　处：《中华口腔医学杂志》2011年第5期276-279,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30860314）;新疆维吾尔自治区自然科学基金（200821112）;新疆维吾尔自治区科技攻关和重点项目（200733146）

摘　　要：目的构建孕鼠慢性牙周炎（chronic periodontitis，CP）模型，探讨肿瘤坏死因子（TNF）在CP孕鼠早产胎盘组织中的表达。方法选取60d龄Wistar雌鼠20只按随机数字表分为两组：正常对照组8只、CP组12只，CP组建立牙周炎模型，4周后两组雌鼠均与雄鼠合笼，观察两组孕鼠的分娩时间及新生鼠出生体质量，至孕19d（大鼠足孕期为21－23d，早于孕19d即为早产）处死全部孕鼠取胎盘组织。观察孕鼠牙周组织的病理改变；实时荧光定量聚合酶链反应（PCR）法分析胎盘组织中TNF的表达。结果实验牙的牙周检查及组织病理学观察结果显示，已成功建立了孕鼠CP动物模型。CP组共有7只孕鼠提前分娩，活产仔鼠30只；正常对照组至孕19d处死时，活产仔鼠56只。CP组早产孕鼠每胎平均活产仔鼠数[（4．1±2．2）只]显著低于正常对照组[（9．2±2．2）只]，CP组仔鼠出生体质量[（5．01±0．43）g]也显著低于正常对照组[（5．79±0．53）g]，P〈0．05。TNF基因在CP组和正常对照组的相对定量表达值分别为（1．807±0．265）、（1．003±0．021），差异有统计学意义（P=0．001）。结论TNF在慢性牙周炎孕鼠胎盘组织中呈过表达，它与孕鼠的早产及仔鼠出生体质量低有一定关系。Objective To examine the expression of tumor necrosis factor in placenta of pregnant rats with chronic periodontitis. Methods Twenty Wistar female rats were randomly divided into two groups, control （n = 8 ） and experimental group （n = 12 ）. The periodontitis model was established in the experimental group. The females and males in the two groups got together four weeks later. Nineteen days after pregnancy all rats were executed and placenta collected. The delivery time and neonatal birth weight were recorded and the pathological changes of periodontal tissue observed. Tumor necrosis factor （TNF） expression was examined in placenta by real-time quantitative polymerase chain reaction analysis. Results The animal model of chronic periodontitis was successfully established. Experimental group delivered 30 offspring and the control group 56 offspring. The average number of pups born alive per litter in experimental group（4. 1± 2. 2） was significantly lower than that in control group（9.2 ±2. 2 ） , P 〈 0. 05. The birth weight of pups in experimental group[ （5.01±0. 43） g] was significantly lower than that in the control groupl （5. 79±0. 53） g], P 〈 0. 05. The relative quantitative expression of TNF was （ 1. 807 ± O. 265 ） in experimental group the and （ 1. 003± O. 021 ） in the control group （ P = 0. 001 ）. Conclusions Chronic periodontitis may be related to preterm low birth weight.

关 键 词：牙周炎 早产 模型 动物 肿瘤坏死因子类 

分 类 号：R781.4[医药卫生—口腔医学]