热凝脑膜中动脉对硝酸甘油致偏头痛大鼠血CGRP和SP含量的影响  

Heat coagulation of middle meningeal artery affects plasma CGRP and substance P levels in migraine rat triggered by nitroglycerin

作　　者：朱晓凤[1] 韩月臣[1] 熊文萍[1] 王海波[1] 李建峰[2] 刘闻闻 樊兆民[1] 

机构地区：[1]山东大学附属省立医院耳鼻咽喉-头颈外科,济南250012 [2]山东省临床医学研究院眼耳鼻喉科学研究所

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第10期460-462,468,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：山东省科技攻关计划课题(No:2007GG20002)

摘　　要：目的:探讨热凝脑膜中动脉对硝酸甘油诱发的偏头痛大鼠静脉血降钙素基因相关肽(CGRP)和P物质(SP)含量的影响。方法:24只健康雌性Wistar大鼠随机分为4组:A组(生理盐水组)为皮下注射生理盐水,B组(硝酸甘油组)为皮下注射硝酸甘油注射液10 mg/kg构建偏头痛动物模型,C组(假手术组)为单纯暴露脑膜中动脉后皮下注射硝酸甘油注射液10 mg/kg,D组(手术组)为热凝脑膜中动脉后皮下注射硝酸甘油注射液10 mg/kg。观察各组大鼠行为学变化及应用放射免疫法(RIA)检测各组大鼠静脉血CGRP和SP含量。结果:A组大鼠偶尔出现挠头现象,D组大鼠与B、C组大鼠相比,挠头及爬笼次数明显减少且频度降低,无耳红现象。4组大鼠静脉血CGRP含量分别为(12.14±4.99)、(57.34±10.27)、(51.40±13.13)和(27.96±8.32)ng/L;SP含量分别为(6.78±2.09)、(20.03±5.74)、(22.33±6.83)和(13.27±2.31)ng/L。B、C及D组大鼠静脉血CGRP和SP含量与A组相比均有增加,差异具有统计学意义,且D组大鼠静脉血CGRP和SP含量低于B和C组大鼠,差异具有统计学意义,但B与C组之间差异无统计学意义(均P>0.05)。结论:热凝大鼠脑膜中动脉可减轻由硝酸甘油诱发的偏头痛症状,这可能是通过抑制CGRP和SP的释放引起的。Objective：To detect the influence of heat coagulation of middle meningeal artery（MMA） on plasma CGRP and SP levels in migraine rat triggered by nitroglycerin（NTG）.Method：Twenty-four female Wistar rats were randomly divided into four groups,with six rats in each group.In group A,rats were treated with isotonic saline;in group B,rats were injected subcutaneously with 10 mg/kg nitroglycerin to set up the animal model of migraine;in group C,rats were subjected to sham surgery by merely exposing MMA and then injected with nitroglycerin（10 mg/kg）;and in group D,rats were firstly subjected to heat coagulation of MMA and,subsequently,injected with nitroglycerin（10 mg/kg）.Following the injection,the animal＇s behavior was continuously recorded by an observer.Radioimmunoassay was employed to measure the amounts of CGRP and SP in venous plasma.Result：Injection of 10 mg/kg nitroglycerin was able to trigger the animal model of migraine as evidenced by the appearances of a series of symptoms,such as,scratching head frequently,climbing cage and red ear.Rats in group A occasionally showed off scratching head,whereas,rats from group B and group C presented with much severe symptoms.Of note,rats in group D appeared slightly scratching head and climbing cage,without red ear compared with group B and C.At the same time,the amounts of plasma CGRP and SP in group B,group C,or group D were increased significantly compared with that of group A（P0.05）.Furthermore,the plasma CGRP and SP levels in group D were much lower than that in group B and group C（P0.05）,but there was no statistical difference between group B and group C（P0.05）.Conclusion：Heat coagulation of MMA may relieve symptoms of rats following nitroglycerin infusion,possibly by inhibiting the release of CGRP and SP.

关 键 词：偏头痛 脑膜中动脉 降钙素基因相关肽 P物质 

分 类 号：R747.2[医药卫生—神经病学与精神病学]