肝脏组织样本库的建立及管理  

Establishment and management of a liver tissue bank in China

作　　者：陆晓芳[1] 马欣[1] 周骏群[1] 陈凤秋[1] 杨清平[1] 魏明月[1] 刘逸敏[1] 杨远[1] 周伟平[1] 

机构地区：[1]中国人民解放军第二军医大学东方肝胆外科医院,上海市200438

出　　处：《世界华人消化杂志》2011年第11期1187-1190,共4页World Chinese Journal of Digestology

基　　金：国家科技重大专项基金资助项目;No.2008ZX10002-018~~

摘　　要：目的:建立按照统一标准收集的,具备完整临床资料的肝脏组织及相关体液的肝脏组织样本库.方法:制定标准化操作流程,采集患者的血液、尿样和新鲜和石蜡手术样本,血样本包括术前术后样本以及患者随访的血样本,按不同要求处理分装,应用数据库管理系统,建设临床资料完整的组织样本库.结果:收集2009-03/2010-124000余例完整肝脏肿瘤新鲜组织、石蜡切片、血样、尿样,及完整的临床资料及随访资料,包括了超过2500例原发性肝细胞癌,及约1500例其他肝脏肿瘤,广泛应用于肝脏病学研究的各个领域.结论:建立了规范化、规模化的肝脏组织样本库,为肝癌及其他肝脏病学的研究提供高质量的样本.AIM：To develop a standardized and well-rounded liver tissue bank available for hepatol-ogy research using well-characterized and optimally preserved liver tumor tissue samples.METHODS：Standardized operational procedures were developed,and samples such as fresh-frozen surgical tissue samples,pre-and postoperative whole blood,urine and paraffin section samples were collected.Using the National Liver Tissue Bank（NLTB）data management platform,the database can be accessed and renewed automatically or manually with the Hospital Information System（HIS）.RESULTS：From Mar 2009 to Dec 2010,over 4000 individuals had been enrolled as liver tumor donors to the NLTB,including 2 500 cases of newly diagnosed hepatocellular carcinoma（HCC）,and 1 500 cases of diagnosed begin or malignant liver tumors.Database access and sample preservation can be managed easily and correctly with the data management platform.CONCLUSION：We established a standard liver tissue bank using high-quality well-characterized samples,which will become the cornerstone for hepatology research,especially the diagnosis and treatment of HCC and other liver diseases.

关 键 词：肝肿瘤 组织库 信息系统 标准化 

分 类 号：R735.7[医药卫生—肿瘤]