三种蛋白在上皮性卵巢癌中的表达及意义  

The expression and clinical significance of PIK3CA, AKT2 and PTEN in epithelial ovarian cancer

作　　者：白月[1] 张林燕[1] 

机构地区：[1]内蒙古医学院附属医院妇产科,呼和浩特010050

出　　处：《中国医药》2011年第6期727-730,共4页China Medicine

摘　　要：目的检测PIK3CA、AKT2、PTEN在上皮性卵巢癌中的表达情况，分析其与临床病理参数的关系，探讨三者在上皮性卵巢癌发生发展中的可能作用机制。方法采用免疫组织化学S-P法及半定量分析法检测29例正常卵巢及64例上皮性卵巢癌组织中PIK3CA、AKT2、PTEN蛋白的表达情况，分析在上皮性卵巢癌中三者与临床病理特征之间的关系以及三者之间的相关性。结果PIK3CA蛋白表达部位主要在细胞质内。在正常卵巢组织及上皮性卵巢癌组织中灰度值分别为（85．21±4．74）、（46．68±10．22），差异有统计学意义（P〈0．01）。AKT2蛋白表达部位主要在细胞质内。在正常卵巢组织及上皮性卵巢癌组织中灰度值分别为（85．72±4．29）、（46．17±9．29），差异有统计学意义（P〈0．01）。PTEN阳性染色主要定位于细胞质，有少量定位于细胞核。在正常卵巢组织及上皮性卵巢癌组织中灰度值分别为（41．49±4．67）、（73．74±11．33），差异有统计学意义（P〈0．01）。PIK3CA与AKT2蛋白在上皮性卵巢癌中的表达均高于正常卵巢组织，PTEN蛋白表达低于正常卵巢组织，差异均有统计学意义（P〈0．01）；上皮性卵巢癌中，PIK3CA与AKT2蛋白在中低分化及Ⅲ～Ⅳ期组织中表达高于高分化及Ⅰ～Ⅱ期，差异有统计学意义（P〈0．05），PTEN蛋白在中低分化及Ⅲ～Ⅳ期组织中表达低于高分化及Ⅰ～Ⅱ期，差异有统计学意义（P〈0．05），三者在患者的年龄、组织学类型分组中表达差异无统计学意义（P〉0．05）；上皮性卵巢癌中，PIK3CA与AKT2蛋白表达呈正相关（r=0．674，P〈0．01），PIK3CA与PTEN表达呈负相关（r=-0．514，P〈0．01），AKT2与PTEN表达呈负相关（r=-0．589，P〈0．01）。结论PIK3CA、AKT2蛋白的表达升高和PTEN蛋白表达降低在卵巢癌的发生、发展中起重要作用，P13K／AKT信号通路参与卵巢癌的演Objective To investigate the expression of PIK3CA, AKT2 and PTEN in epithelial ovarian cancer, to analyze their relation to clinical and pathologic features and prognosis of epithelial ovarian cancer. Methods The expression of PIK.3CA, AKT2 and PTEN protein were studied by immunohisto chemical methods in 29 normal ovary tissues and 64 epithelial ovarian carcinoma. The correlation between clinical and pathologic features in ovarian cancer was also analyzed. Results The expression of PIK3CA and AKT2 protein in epithelial ovarian cancer was significantly higher than that in normal ovary tissues （ P 〈 0.01 ）. In epithelial ovarian cancer, the expression of PIK3CA and AKT2 protein in mid and low histological grade and Ⅲ-Ⅳ clinical stage was significantly higher than that in high histological grade and Ⅰ- Ⅱ clinical stage（P 〈 0.05）. In epithelial ovarian cancer, the expressions were not related with age and histological type（P 〉 0.05 ）. The expression of PIK3CA protein was positively correlated with the expression of AKT2 protein（r = 0.674, P 〈 0.01 ）. The expression of PTEN was negatively correlated with the expression of PIK3CA （r = -0.514, P 〈 0.01 ）and the expression of PTEN was negatively correlated with the expression of AKT2 （ r = - 0. 589, P 〈 0.01 ）. Conclusions The high expression of PIK3CA and AKT2 protein as well as the low expression of PTEN protein are closely related to the occurrence and development of ovarian cancer. PI3K/AKT signal pathway takes part in the evolvement process of ovarian cancer. To investigate the expression of PIK3CA, AKT2 and PTEN protein are helpful for the clinical diagnosis and treatment of ovarian cancer.

关 键 词：上皮性卵巢癌 免疫组织化学 临床病理特征 

分 类 号：R737.31[医药卫生—肿瘤]