共刺激分子CD86在急性髓性白血病细胞的表达及其临床意义  

The expression of costimulatory molecules CD86 in acute myelogenous leukemic cells and clinical im-plication

作　　者：余美霞[1] 周咏明[2] 邱宇珍[2] 姚春红[2] 周浩[3] 

机构地区：[1]武汉科技大学附属天佑医院妇产科,武汉430064 [2]九江学院附属医院妇产科血液科 [3]华中科技大学同济医学院附属协和医院血液科

出　　处：《中国医师杂志》2011年第4期437-440,共4页Journal of Chinese Physician

基　　金：江西省自然科学基金资助项目，湖北省教育厅资助基金，武汉科技大学附属天佑医院中青年基金项目，武汉科技大学专项基金项目

摘　　要：目的探讨共刺激分子CD80、CD86在急性髓性白血病细胞中的表达及其临床意义。方法采用细胞培养白血病细胞（HL60，U937，NB4及I（562）及流式细胞仪检测共刺激分子CD80、CD86在急性髓性白血病患者骨髓细胞中的表达及其在急性髓性白血病细胞HL-60细胞、U937细胞、NB4细胞和K562细胞的表达。结果CD80在急性髓性白血病骨髓细胞中的表达很低或不表达，CD86在急性髓性白血病骨髓细胞表达[（27．86±19．65）％]高于对照组[（1．21±0．13）％，t=3．55，P〈0．01]。其中CD86在M4型（48．65±21．92）％、M5型（39．25±18．67）％表达较高，但与对照组单核细胞表达（50．204-20．31）％比较差异均无统计学意义（P〉0．05）。CD86在HL-60细胞、U937细胞和NB4细胞的培养24h后表达分别为（30．624-5．35）％、（24．12±5．23）％、（21．25±3．78）％，与培养48h后表达[（29．43±4．67）％、（26．564-6．54）％、（23．21±6．98）％]比较差异均无统计学意义（P〉0．05）。CD80在HL一60细胞、U937细胞和NB4细胞的表达很低或不表达，K562细胞不表达共刺激分子CD80和CD86。结论CD86在HL-60细胞、U937细胞和NB4细胞和急性髓性白血病患者骨髓细胞中表达。Objective To investigate costimulatory molecules CD80 and CD86 expression in acute myelogenous leukemic cells and clinical implication. Methods The expression of CD80 and CD86 in the patients with acute myelogenous leukemia and HL-60 cells, U937 cells, NtM cells, K567 cells was confirmed by Flow Cytometer. Results CD80 was very low or no expression in patients with acute myelogenous leukemia. CD86 expressed in acute myelogenous leukemia （27.86 ± 19. 65）%, which was much higher than that in control group[ （1.21±0. 13 ）%, t = 3.55, P 〈0. 01 ]. No significant changes were observed in the expression of CD86 in M4 cells（48.65 ＋21.92）%, M5 cells（39.25 ± 18.67）% and control group （50. 20 ± 20. 31）% （ P 〉0.05）. After the cells were cultured for 24 h and 48 h, the expression of CD86 was （30.62±5.35）% and （29.43±4.67）% in HL-60 ceils , （24. 12 ± 5.23）% and （26.56 ± 6. 54）% in U937 cells, and, （21.25 ± 3.78）% and （23.21 ± 6. 98）% in NtM cells （allP 〉0.05）. The expression of CD80 and CD86 was very low in K562 cells. Conclusions Costimulatory molecules CD86 ex- pressed in acute myelogenous leukemic cells in the patients with acute myelogenous leukemia and HL-60 cells, U937 cells, NIM cells.

关 键 词：抗原 CD86/代谢 白血病 髓样 急性/代谢 

分 类 号：R733.71[医药卫生—肿瘤]