引种栽培黄芪药材中主要黄酮类成分含量比较研究  

Comparison of the Content of Major Flavonoids in the Introduced and Cultivated Radix Astragali

作　　者：王雪洁[1,2] 孙海峰[1,2] 谢道生[1] 武滨 秦雪梅[2] 

机构地区：[1]山西大学化学化工学院,太原030006 [2]山西大学中医药现代化研究中心,太原030006 [3]山西省医药集团有限责任公司,太原030012

出　　处：《植物研究》2011年第3期358-362,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(NO.2006BA106A15-7)

摘　　要：种质资源的筛选和评价对于黄芪的规范化栽培与种植具有重要意义。本研究即分别采用分光光度法、高效液相色谱法测定引种至山西浑源黄芪道地产区不同来源黄芪药材中总黄酮和毛蕊异黄酮、芒柄花素的含量,并进行相关性分析。结果表明:上述成分不仅仅与黄芪栽培方式、产地有关,还与种质来源密切相关;山西、陕西地区黄芪种质适宜在当地栽培和野生种植,而甘肃、内蒙古黄芪引种到山西浑源地区黄酮类物质含量较原产地为高,适宜大面积引种;黄芪药材中毛蕊异黄酮和芒柄花素的含量呈显著的正相关。该研究结果将为黄芪的规范化种植、优良品种选育及质量评价体系的优化提供新的科学依据。Screening and evaluating the germplasm resources is important for the standardization of cultivation and planting of Radix Astragali（RA）.In this study,UV-Vis spectrophotometry and HPLC were used to determine the contents of total flavonoids,calycosin and formononetin in various RAs collected from different sources introduced to Shanxi.The results showed that the contents of the above components were not only related to their culture styles and origins,but also closely related to the germplasms.Astragalus germplasm from Shanxi and Shaanxi were suitable to be widely planted in the local regions,while those introduced from Gansu,Inner Mongolia,and Shanxi to Hunyuan,Shanxi had a higher degree of flavonoids than those from their corresponding original regions,hence were suitable for large introduction.The correlation analysis further showed that the level of calycosin was significantly correlated to the level of formononetin.The study will be informative to the standardization planting,excellent breed screening,and optimization of quality evaluation system of the medical plant.

关 键 词：黄芪 引种栽培 质量评价 黄酮类物质 

分 类 号：S567.2[农业科学—中草药栽培]