响应面法优化酶解辅助提取翅果油树叶总黄酮  

Optimization of Enzyme-assisted Extraction of Total Flavonoids from Elaeagnus mollis Diels Leaves by Using Response Surface Methodology

作　　者：郭彩珍[1] 薛丹[1] 侯学敏[1] 张直峰[1] 闫桂琴[1] 

机构地区：[1]山西师范大学生命科学学院,生物多样性研究所,临汾041004

出　　处：《植物研究》2011年第3期371-376,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30470296);山西省自然科学基金项目(2008011058-1)

摘　　要：利用酶辅助法对翅果油树(Elaeagnus mollis Diels)叶中总黄酮的提取工艺进行优化。在单因子试验结果的基础上,采用响应面法(RSM)研究了酶用量、酶解时间、pH值和酶解温度对翅果油树叶总黄酮提取得率的影响。结果表明:纤维素酶具有较好的酶解能力,当酶用量为6.5 mg.g-1,酶解时间为1.5 h,pH4.7,温度为46℃时,总黄酮得率达到7.1%。The process of enzyme-assisted extraction of total flavonoids from the leaves of Elaeagnus mollis Diels was optimized.Based on the single-factor tests,the effects of enzyme amount,time of enzymatic hydrolysis,pH value and temperature of enzymatic hydrolysis on the extraction yields of total flavonoids from E.mollis Diels leaves were studied by response surface methodology（RSM）.The results indicated that cellulase showed better ability of enzymatic hydrolysis.The optimum enzyme-assisted extraction process was： enzyme amount 6.5mg·g-1,time 1.5h,pH value 4.7 and temperature 46℃.The highest yield of total flavonoids was 7.1%.

关 键 词：翅果油树叶 总黄酮 酶解 响应面 

分 类 号：S567.1[农业科学—中草药栽培]