白藜芦醇的光稳定性和热稳定性研究  

Study of photo-stability and thermo-stability of resveratrol

作　　者：史先敏[1,2] 严泽民[2] 谢静红[2] 周华锋[2] 何宏轩[3] 段明星[1] 

机构地区：[1]清华大学生命科学学院生物膜与膜生物工程国家重点实验室,北京100084 [2]江苏隆力奇生物科技股份有限公司研发中心,江苏常熟215555 [3]中国科学院动物研究所,北京100101

出　　处：《日用化学工业》2011年第3期204-207,共4页China Surfactant Detergent & Cosmetics

摘　　要：采用分光光度法和高效液相色谱法(HPLC)对不同环境条件下的白藜芦醇的稳定性进行了研究,并对不同环境条件处理后的样品进行了清除二苯代苦味酰基自由基活性和抑制酪氨酸酶活性的研究。结果表明,反式白藜芦醇具有光不稳定和热不稳定性,在光照和/或加热下有部分转变为顺式白藜芦醇。样品光照0.5 h后,306 nm处吸光度值下降52%;60℃条件下放置60 h后,306 nm处吸光度值下降32%。未经光照的新配制的白藜芦醇溶液对单酚酶和双酚酶的抑制率为零,而光照36 h的白藜芦醇溶液对单酚酶和双酚酶的抑制率分别达到30.74%和19.42%,并且其自由基清除率比新配制的样品增强了约300%。The photo-stability and thermo-stability of resveratrol were investigated using ultraviolet-visible spectrophotometry and high performance liquid chromatography.Meanwhile the activities for scavenging 1,1-dipheny-2-picryhydrazyl free radical（DPPH·） and inhibiting rate to mushroom tyrosinase of the resveratrol after being treated by sun exposure and heating in water were also studied.The results indicated that trans-resveratrol is unstable under sun exposure and heating.It changes partly to cis-resveratrol under the action of sun light and/or heating.The absorbance of 306 nm decreases 52% after sun exposure for 0.5 h and decreases 32% after heating for 60 h at 60 ℃.Under sun exposure for 36 h,the scavenging capacity for DPPH· of resveratrol solution increases 300% as comparing with the original resveratrol solution;and the inhibition rate to phenolase and catecholase of the resveratrol solution after sun exposure achieves 30.74% and 19.42% respectively,while that of the original resveratrol solution before sun exposure is zero.

关 键 词：化妆品添加剂 白藜芦醇 光稳定性 热稳定性 DPPH自由基 酪氨酸酶 

分 类 号：R284.1[医药卫生—中药学]