激光捕获显微切割技术联合短串联重复序列多态性分析在葡萄胎受精类型鉴别中的应用研究  

Combination of laser capture micro-dissection(LCM) and short tandem repeats(STR) polymorphisms in determination of fertilization types of gestational trophoblastic disease

作　　者：李晓倩[1] 杨静[2] 杨邵敏[3] 齐伟宏[4] 王静[2] 袁杨[1] 郝婷[1] 孙佳良[5] 张小为[1] 

机构地区：[1]北京大学第三医院妇产科,100191 [2]北京海淀区妇幼保健院 [3]北京大学医学部病理学系 [4]北京医院妇产科 [5]辽宁医学院附属第一医院妇产科

出　　处：《中国优生与遗传杂志》2011年第6期16-18,117,共4页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金资助(编号30772321)

摘　　要：目的探讨激光捕获显微切割技术(LCM)联合短串联重复序列(STR)多态性分析用于葡萄胎受精类型鉴别的可行性。方法首先通过LCM获得葡萄胎病病理组织的滋养细胞,再用16个位点(D8S1179,D21S11,D2S1338,CSF1PO,TH01,TPOX,D3S1358,D13S317,D16S319,D19S433,FGA,D18S51,D5S818,VWA,D7S820,Am)复合微卫星序列-PCR方法对61例病理学诊断为葡萄胎标本进行分析,明确其受精类型。结果用LCM技术从61例标本中获得滋养细胞后,成功提取DNA46例,浓度范围为:6.2-114.5(ngμ/l)。经STR多态性分析确定空卵单精子受精完全性葡萄胎13例,空卵双精子受精完全性葡萄胎13例,部分性葡萄胎20例。结论 LCM联合多重STR多态性分析能够确定葡萄胎的受精类型。Objective：To investigate the feasibility of short tandem repeats（STR） polymorphisms with Laser capture micro-dissection（LCM） in determination of fertilization types of hydatidiform mole（HM）.Methods： The pure trophoblastic cells of HM were obtained by LCM and all the specimens were examined by multiplex STR—PCR（16loci： D8S1179,D21S11,D2S1338,CSF1PO,TH01,TPOX,D3S1358,D13S317,D16S319,D19S433,FGA,D18S51,D5S818,VWA,D7S820,Am）,then to determine the fertilization types of HM.Results： the numbers that we successfully extracted DNA from all the 61 cases by LCM were 46 cases,the range of DNA＇s concentration： 6.2-114.5（ng/μl）.And through the analysis of STR-PCR we found that complete hydatidiform moles（CHM） from fertilization of an anuclear empty ovum by a haploid sperm were 13 cases,while complete hydatidiform moles（CHM） from dispermic fertilization were 13 cases,and the remain 20 cases were partial hydatidiform moles（PHM）.Conclusion： The results suggested that short tandem repeats（STR） polymorphisms with laser capture micro-dissection can determine the fertilization types of HM.

关 键 词：葡萄胎(HM) 激光捕获显微切割技术(LCM) 短串联重复序列(STR) 等位基因 

分 类 号：R737.33[医药卫生—肿瘤]