赖氨酰氧化酶对胃癌细胞HGC-27体外迁移黏附能力的影响  

Effect of lysyl oxidase on migration and adhesion of human gastric cancer HGC-27 cells in vitro

作　　者：马佰菁[1] 黄菱[1] 韩梅[1] 王玉巧[2] 赵建宁[1] 孙伯坚[1] 林佳静[1] 宋婷阁[1] 

机构地区：[1]宁夏医科大学病原生物学与免疫学系,银川750004 [2]宁夏人民医院ICU

出　　处：《中华肿瘤杂志》2011年第5期331-333,共3页Chinese Journal of Oncology

基　　金：基金项目：宁夏自然科学基金（NZ1095）;宁夏高等学校科学研究项目（2009-47）

摘　　要：目的 研究赖氨酰氧化酶（LOX）对胃癌细胞HGC-27体外迁移和黏附能力的影响.方法 体外培养胃癌细胞HGC-27,以不同浓度的B-氨基丙腈（BAPN）抑制LOX的活性.采用体外划痕实验检测对细胞迁移能力的影响,采用同质黏附和异质黏附实验分析对细胞黏附能力的影响.结果 当BAPN浓度为0.2 mmol/L时,胃癌细胞的体外迁移能力在8、24、32、48 h各时间点均较未加BAPN下降（P〈0.05）;且HGC-2q细胞在同质黏附介质上生长60 ndn后,同质黏附细胞数为（7.78±0.11）×103个/ml,与对照组[（6.97±O.07）×103个/ml]比较,差异有统计学意义（P〈0.05）;异质黏附细胞数为（8.35±0.10）×103个/ml,与对照组[（8.98±0.15）×103个/ml]比较,差异有统计学意义（P〈0.05）.当BAPN浓度为0.3 mmol/L时,胃癌细胞的体外迁移能力在各时间点均较未加BAPN下降,同质黏附细胞数增加至（8.02±0.11）×103个/ml,异质黏附细胞数减少至（7.93±0.07）×103个/ml,与对照组或0.2 mmol/L BAPN组比较,差异均有统计学意义（P〈0.05）.结论 LOX可能通过促进肿瘤细胞的迁移和异质黏附能力,降低其同质黏附能力,促进肿瘤的转移.Objective To study the effects of lysyl oxidase （ LOX） on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro. Methods The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile （BAPN）. The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments. Results Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from （6.97 ± 0.07） x l03/ml to （7.78±0.11） ×103/ml; and the number of cells with heterogeneous adhesion was decreased from （8.98± 0. 15） x 103/ml to （8. 35 ±0. 10） ×103/ml, both 〈0. 05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to （8. 02 ± 0. 11） ×103/ml and the number of cells with heterogeneous adhesion was down to （7.93 ±0.07） ×103/ml （P 〈 0. 05）. Conclusion LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion.

关 键 词：胃肿瘤 赖氨酰氧化酶 细胞迁移 细胞黏附 肿瘤转移 

分 类 号：R735.2[医药卫生—肿瘤]