榆木蠹蛾性信息素通讯系统的超微结构观察  

Ultrastructural observation of the sex pheromone communication system in Holcocerus vicarius(Walker)(Lepidoptera:Cossidae)

作　　者：杨美红[1] 张金桐[1] 范丽华[1] 刘红霞[2] 骆有庆[3] 宗世祥[3] 曹川健 

机构地区：[1]山西农业大学化学生态研究所,山西太谷030801 [2]山西农业大学信息科学与工程学院,山西太谷030801 [3]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [4]宁夏森林病虫防治检疫总站,银川750004

出　　处：《昆虫学报》2011年第5期522-530,共9页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31070581);国家"十一五"科技支撑计划课题(2006BAD08A10)

摘　　要：为了解榆木蠹蛾Holcocerus vicarius（Walker）雌蛾性信息素分泌腺（性信息素释放系统）位置、表面形态和超微结构及雄蛾触角感受器（性信息素接收系统）的种类、形态、分布及功能,利用扫描电镜和透射电镜对榆木蠹蛾雌蛾性信息素分泌腺和雄蛾的触角进行观察。结果表明：榆木蠹蛾雌蛾性信息素分泌腺位于腹尖末端第8~9节节间膜上的背面中央区域,腺体表面分布着许多饱满的锥状突起,2日龄处女雌蛾腺体细胞间有明显的胞连接,细胞基底膜基褶较多,质膜上分布着微绒毛,并与内表皮连接,内表皮上含有多层几丁质,胞质中含有脂质粒、大量空泡、光面内质网、粗面内质网及线粒体;雄蛾触角鞭节上有5种感受器,为毛形感器、刺形感器、锥形感器、腔锥形感器和曲毛形感器,其中毛形感器数量最多,曲毛形感器最少。柄节和梗节被大量鳞片覆盖,未观察到感器。榆木蠹蛾性信息素通讯系统的研究为榆木蠹蛾性信息素的生物合成、性信息素的提取、鉴定及成虫生殖交配生物学行为提供了依据。The aim of the study was to understand the location,surface conformation and ultrastructure of the sex pheromone glands（the release system of sex pheromone） of female moth,and type,configuration,distribution and function of male antennae sensilla（sex pheromone receiving system） of Holcocerus vicarius（Walker） adult.Sex pheromone glands of the females and the antennae of the males were observed by using scanning electron microscope（SEM） and transmission electron microscope（TEM）.The results showed that the pheromone glands were formed at the mid-dorsal region of the intersegmental membrane between the 8th and 9th abdominal segments.Many plump cones were distributed on the surface of the gland.There was evident conjugation between glandular cells and there were many basal involutions in the basal membrane of cells.Microvilli were distributed on the cytoplasmic membrane and linked with endocuticle on which there were many layers of chitin.In the glandular cytoplast of 2-day-old virgin female moths,the cells contained lipid granules,a lot of empty bubbles,smooth and rough endoplasmic reticulum,and mitochondria.There were five types of sensilla on male antenna flagellum,including sensillum trichodea,sensillum chaetica,sensillum basiconica,sensillum coeloconica and sensillum trichodea curvata,among which sensillum trichodea is the most abundant and sensillum trichodea curvata is the least abundant.The sensilla were not observed on the antenna scape and pedicel for they were covered by a large number of scales.The study provides the basis for understanding the biosynthesis of sex pheromone,extraction and identification of sex pheromone,and reproductive and mating behavior of H.vicarius.

关 键 词：榆木蠹蛾 性信息素 通讯系统 超微结构 扫描电镜 透射电镜 

分 类 号：Q964[生物学—昆虫学]