川芎嗪对氧诱导视网膜病变中视网膜神经细胞凋亡的抑制作用  

Tetramethylpyrazine inhibits retinal cell death in oxygen-induced retinopathy

作　　者：梁小玲[1] 周焕娇[1] 杨诚[1] 孙刚[1] 张熙芳[1] 魏丽清[1] 

机构地区：[1]中山大学中山眼科中心、眼科学国家重点实验室,广州510060

出　　处：《中华眼底病杂志》2011年第3期271-274,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金（30973899）;广东省科技计划项目（20098060700106）

摘　　要：目的观察川芎嗪（TMP）对氧诱导视网膜病变（OIR）中视网膜神经细胞凋亡的抑制作用。方法48只C57BL／6新生幼鼠随机分为正常对照组、0IR对照组及OIR药物组，分别为18、18、12只。正常对照组在正常空气环境中饲养。OIR对照组、OIR药物组幼鼠于出生后7d置于含氧体积分数（75±3）％的氧气箱中连续生活5d；出生后12d，幼鼠回到正常空气环境中饲养，建立OIR模型。出生后12～16d，OIR药物组按200mg／kg的剂量腹腔注射TMP，1次／d；正常对照组、0IR对照组同时注射相同体积的含0．1％二甲基亚砜（DMs0）的生理盐水。出生后12、14、17d，摘除幼鼠眼球作石蜡切片并行苏木精-伊红（HE）和脱氧核苷酸末端转移酶介导缺口末端标记（TuNEL）染色。观察视网膜无灌注区形态学变化，检测视网膜神经细胞凋亡数量。结果正常对照组幼鼠视网膜各层结构清晰。出生后12d，OIR对照组幼鼠视网膜内核层（INL）可见少量染色质凝聚的细胞核。出生后14d，OIR对照组幼鼠视网膜INI。可见大量染色质凝聚的细胞核，OIR药物组幼鼠视网膜INI。均可见少量染色质凝聚的细胞核。出生后17d，OIR对照组幼鼠视网膜INL、内丛状层（IPL）和外丛状层（OPL）厚度变薄；OIR药物组幼鼠视网膜中周部INL、IPL和OPL厚度较正常对照组薄，较0IR对照组厚。出生后12d，OIR对照组幼鼠视网膜TUNEL阳性细胞数量为正常对照组的6倍，差异有统计学意义（t=9．432，P〈0．001）。出生后14d，3组幼鼠视网膜TUNEL阳性细胞数量比较，差异有统计学意义（F=587．217，P〈0．001）；OIR对照组幼鼠视网膜TUNEL阳性细胞数量为正常对照组的28倍，差异有统计学意义（t=49．813，P〈0．001）；OIR药物组幼鼠视网膜TuNEL阳性细胞数量较0IR对照组减少了82．3％，差异有统计学意义（t=42．434，P〈0．001）。出生后17d，3组幼鼠视网膜TUNEL�Objective To observe the inhibitory effect of tetramethylpyrazine （TMP） on apoptosis of retinal neurons in oxygen-induced retinopathy （OIR）. Methods 48 C57BL/6 mice were randomly divided into normal control group （n= 18）, OIR control group （n= 18） and OIR TMP group （n= 12）. The mice of normal control group were raised in room air. From the postnatal day 7 （P7）, mice of the other two groups were exposed to （75 3） % oxygen for 5 days and then returned to room air to establish OIR model. The mice of OIR TMP group received intraperitoneal injection of TMP （200 mg/kg） once a day from P12 to P16, meanwhile the mice of normal control group and OIR control group were injected with the same volume of normal saline containing of 0. 1%DMSO. At P12, P14 and P17, the morphologic changes in retinal avascular zone and the number of retinal apoptotic cell were observed by HE staining and TUNEL assay. Results At P12, there were a few of chromatin condensation and pycnic nuclei in the inner nuclear layer （INL） of OIR control group. At P14, a great quantity （OIR control group） or some （OID TMP treated group） chromatin condensation and pycnic nuclei in the central INL were observed. At P17, the thickness of INL, inner plexiform layer （IPL） and outer plexiform layer （OPL） in the OIR control group were reduced; the thickness of INL, IPL and OPL in the OIR TMP group weas thinner than those in the normal control group and thicker than those in the OIR control group. At P12, the TUNEL-positive cells in the OIR controlgroup was 6 times of the normal control group （F= 587. 217, P〈0. 001）. At P14, the difference of TUNEL-positive cells in three groups was significant （F= 587. 217, P〈0. 001）; the TUNEL-positive cells in the OIR control group was 28 times of the normal control group （t=49. 813,P〈0. 001）; the TUNEL- positive cells in the OIR TMP group has reduced 50% compared with the OIR control group （t=42. 434, P〈0. 001）. At P17, there was no significant difference

关 键 词：视网膜疾病/药物疗法 川芎嗪/治疗应用 细胞凋亡/药物作用 动物实验 

分 类 号：R774.1[医药卫生—眼科]