钙超载对PC12细胞活性及p-CREB表达的影响  

Effects of Ca^(2+) Overload on Viability of PC12 Cells and Expression of p-CREB

作　　者：孙洲[1] 龚瑜[2] 孙圣刚[1] 苏颖[1] 田橙[3] 梁直厚[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,湖北武汉430022 [2]湖北省新华医院内二科,湖北武汉430015 [3]中国船舶重工集团公司第七一二研究所卫生科,湖北武汉430064

出　　处：《武汉大学学报（医学版）》2011年第3期284-286,299,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30901386)

摘　　要：目的:探讨氯化钾(KCl)所致大鼠肾上腺嗜铬细胞瘤细胞(PC12)内钙超载对其细胞活性及磷酸化cAMP反应原件绑定蛋白(p-CREB)表达的影响。方法:将PC12细胞随机分为对照组与干预组,对照组不作任何药物干预,干预组予以KCl 100 mmol/L干预10 min。用MTT法检测两组细胞活性,应用Western blot检测PC12细胞内蛋白激酶A(PKA)活性及p-CREB的表达。结果:MTT法检测细胞活性,KCl组较对照组细胞活性下降,有统计学意义(P<0.05);应用Western blots检测,KCl组PKA活性较对照组明显降低;KCl组p-CREB表达较对照组明显降低。结论:KCl所致钙超载对PC12活性有明显的毒性作用,降低PKA的活性,减少CREB的激活。Objective： To explore the effects of Ca2＋ overload induced by KCl on viability of PC12 cells and expression of p-CREB.Methods： PC12 cells were divided into two groups randomly as control group and KCl group.There was no intervention in control group,while KCl group were given KCl 100 mmol/L for 10 min.Cell viability was determined by MTT assay.PKA activity and expression of p-CREB in PC12 cells were detected by Western blot.Results： The cell viability and PKA activity was reduced in KCl group（P0.05,versus control group）.The expression of p-CREB in KCl group was less than in control group.Conclusion： Ca2＋ overload by KCl was toxic to PC12 cells obviously,and it can cause down-regulation of PKA activity and reduce activated CREB.

关 键 词：钙超载 蛋白激酶A PC12细胞 阿尔茨海默病 

分 类 号：R742.89[医药卫生—神经病学与精神病学]