大肠锯齿状腺瘤和腺癌组织HIF-1α表达及其与VEGF和血管密度相关性的探讨  

Relationship of HIF-1α protein expression with vascular endothelial growth factor and microvessel density in colorectal serrated adenoma and colorectal cancer

作　　者：江鹏飞 丁世华[2] 桂水清 王鹰 邓若愚 

机构地区：[1]深圳市龙华人民医院内科,广东深圳518109 [2]深圳市第二人民医院消化科,广东深圳518035

出　　处：《中华肿瘤防治杂志》2011年第5期348-350,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：深圳市科技局项目(200903173)

摘　　要：目的:探讨大肠锯齿状腺瘤和腺癌组织HIF-1α的表达及其与VEGF和血管密度的关系。方法:采用免疫组化法检测HIF-1α和VEGF蛋白的表达。用CD34单克隆抗体标记血管内皮细胞并计数。结果:锯齿状腺瘤HIF-1α蛋白阳性率(62.5%)显著高于正常大肠黏膜组(13.3%),χ2=15.78,P<0.01;显著低于大肠癌组(85.0%),χ2=4.8,P=0.028。锯齿状腺瘤VEGF蛋白阳性率(53.1%)显著高于正常大肠黏膜组(10.0%),χ2=13.17,P<0.01;而与大肠癌组(72.5%)比较差异无统计学意义,χ2=2.89,P=0.089。HIF-1α表达与VEGF正相关(r=0.317,P=0.041);与MVD正相关,r=0.468,P=0.02。结论:HIF-1α促进肿瘤血管生成,参与大肠锯齿状腺瘤癌变。OBJECTIVE： To explore the relationship of hypoxia-inducibIe factor alpha protein expression with vascular endothelial growth factor and microvessel density in colorectal serrated adenoma and colorec tal cancer. METHODS： HIF-1α and VEGF protein expression were determined by immunohistochemisty in colorectal serrated adenoma and colorectal cancer. Microvessel density was determined by anti-CD34 immunostaining. RESULTS： Positive expression rate of HIF-1α protein in colorectal serrated adenoma group was significantly higher than that in normal group （62.5% vs 13.3%, 7.2 =15. 78,P〈0.01）, but positive expression rate of HIF-1α protein in colorectal serrated adenoma group was significantly lower than that in colorectal cancer. （62.5% vs 85.0G, 72= 4. 8, P= 0. 028）. Positive expression rate of VEGF protein in eolorectal serrated adenoma gruop was significantly higher than that in normal group （53.1% vs 10. 0%, 7.2 =13.17, P〈0.01） and was no difference in colorectal cancer （53.13G vs 72.5%, X^2. 89,P=0. 089）. The expression of HIF-1α protein was positively correlated with the VEGF protein expression and MVD. （r=0. 317,P=0. 041 ; r=0. 468,P=0. 02）. CONCLUSION： Tumor anglogenesis is induced by HIF-1α, and HIF-1α involve in the transforming from colorectal serrated adenoma to colorectal cancer.

关 键 词：大肠肿瘤 缺氧诱导因子-1Α 血管表皮生长因子 

分 类 号：R735.34[医药卫生—肿瘤]