喉鳞癌组织Caveolin-1表达及其与HPV病毒感染相关性的研究  

Expression of Caveolin-1 and its correlation with HPV infection in Laryngeal squamous cell carcinoma

作　　者：肖颖[1,2] 马超[3] 潘新良[3] 阎世坤[1] 曹倩[1] 周亚滨[1,2] 

机构地区：[1]山东大学医学院分子医学实验室,山东济南250012 [2]山东大学医学院病原生物学研究所,山东济南250012 [3]卫生部耳鼻喉科学重点实验室,山东济南250012

出　　处：《中华肿瘤防治杂志》2011年第6期401-404,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30772411)

摘　　要：目的:研究喉鳞状细胞癌组织Caveo-lin-1的表达情况及其与HPV感染之间关系,探讨两者对喉鳞癌生物学行为的影响。方法:分别应用免疫组织化学和原位杂交方法检测喉鳞癌组织中Caveolin-1蛋白及HPV感染的情况,统计学分析两者之间的相关性。结果:Caveolin-1蛋白在喉鳞癌和癌旁组织中的表达率分别为71.2%和9.5%,两者表达差异有统计学意义,P<0.05;Caveolin-1蛋白的表达程度与病理类型、TNM分期、转移相关。喉鳞癌组织HPV感染阳性率为55.1%,癌旁组织为21.4%,两者表达差异有统计学意义,P<0.05;HPV感染与患者年龄、病理类型、TNM分期和转移均无关。喉鳞癌Caveolin-1表达与HPV感染之间呈正相关表达(r=0.62,χ2=10.40,P<0.05)。结论:喉鳞癌组织Caveolin-1表达明显增高并与肿瘤的浸润和转移行为有关,Caveolin-1为判断喉鳞癌生物学的指标之一;HPV感染是喉鳞癌发生的高危因素,并且Caveolin-1与HPV两者之间存在协同作用。OBJECTIVE： To investigate the expression of Caveolin-1 and its correlation with HPV in human Laryngeal squamous cell carcinomas （LSCC） tissues, and analyze their influences on the biological behavior of human LSCC. METHODS： Expressions of Caveolin-1 and HPV16/18 were measured by immunochemical staining or in-situ hybridization in LSCC and Paracancerous tissues. RESULTS： The positive rates of Caveolin-1 in LSCC and Para-cancerous tissues were respectively 71. 2% and 9.5 % （P〈0.05）. Expression rate of Caveolin-1 was significantly associated with tumor differentiation, TNM stage, and metastasis. The frequency of HPV expression in laryngeal carcinoma and Para-cancerous tissues were 55.1% and 21.4% respectively （P〈0. 05） and there was no relation with differentiation and metastasis. The expressions of Caveolin-1 and infection of HPV in the LSCC had a positive correlation（r=0.62 ,X^2= 10.40, P〈0.05）. CONCLUSIONS： Expression of Caveolin-1 is significantly higher in LSCC than that in Para-cancerous tissues and it has significant relations to tumor invasion and metastasis. The pathogenesis of LSCC is closely related with HPV infection. And the mechanism may be that Caveolin-1 mediates the infection of HPV in tumors.

关 键 词：CAVEOLIN-1 喉肿瘤 肿瘤 鳞状细胞 乳头状瘤病毒 人 

分 类 号：R739.65[医药卫生—肿瘤]