新型棉酚衍生物对体外前列腺癌LNCaP细胞自噬影响的研究  

Effect of new gossypol derivative on autophagy of prostate cancer LNCaP cells

作　　者：刘志新[1] 黄晓峰[2] 穆士杰[1] 胡兴斌[1] 詹勇华 张献清[1] 

机构地区：[1]第四军医大学西京医院输血科,陕西西安710032 [2]第四军医大学基础医学部中心实验室,陕西西安710032 [3]西安电子科技大学生命科学院,陕西西安710071

出　　处：《中华肿瘤防治杂志》2011年第6期428-431,465,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究新型棉酚衍生物Apog-ossypolone(ApoG2)在体外对前列腺癌LNCaP细胞的自噬作用。方法:采用MTT、AO染色、透射电镜、流式细胞术和免疫组化方法,研究ApoG2对前列腺癌LNCaP细胞的生长抑制及诱导自噬的作用。结果:当ApoG2浓度>2.5μg/mL时,对前列腺癌LNCaP细胞有明显的增殖抑制,且有时间与剂量依赖性,ApoG2作用LNCaP细胞72 h时的IC50值为4.43μg/mL。AO染色观察LNCaP细胞有自噬特征;透射电镜观察细胞内有自噬泡形成;流式细胞术检测细胞内出现大量酸性液泡细胞器(AVOs);免疫组化结果显示,ApoG2作用于LNCaP细胞后可以使细胞内Beclin 1的表达增高。结论:ApoG2在体外可以诱导前列腺癌LNCaP细胞的自噬,抑制前列腺癌LNCaP细胞的生长。OBJECTIVE： To study the autophagy of prostate cancer LNCaP ceils induced by apogossypolone （ApoG2） in vitro. METHODS： To study the growth inhibition and autophagy of ApoG2 on the prostate cancer LNCaP cells in vitro with MTT assay, AO staining, transmission elctron microscopy, flow cytometry and immunohistochemistry methods. RESULTS：When the concentration of ApoG2 was larger than 2. 5 μg/mL, it had the obvious proliferation inhibition ability to the prostate cancer LNCaP cells in vitro, and it was time and dose dependent. After the calculation, It was found that the IC50 value was 4.43 μg/mL. in 72 h. The obser vation with AO staining indicated that LNCaP cells had the feature of autophagy, The transmission electron microscope observed the appearance of autophagic vacuoles in cells. Using flow cytometry could find the appearance of great lots of AVOs. h indicated that immunohistochemistry could find the Beclin 1 expressions in the tumor cells were obviously increased after ApoG2 treatment. CONCLUSION： The ApoG2 can induce autophagy and inhibit the proliferation of prostate cancer LNCaP cells in vitro.

关 键 词：前列腺肿瘤 棉酚/药理学 棉酚/衍生物 自吞噬作用 

分 类 号：R737.25[医药卫生—肿瘤]