防御素的生物学特性及其抗病基因工程  

Biological characteristics of defensin and its disease-resistance genetic engineering

作　　者：付蓝宝[1,2] 于嘉林[1] 刘伟华[2] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100193 [2]中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《遗传》2011年第5期512-519,共8页Hereditas（Beijing)

基　　金：转基因生物新品种培育重大专项(编号:2008ZX08002-001)资助

摘　　要：防御素是一种富含半胱氨酸的小分子多肽,对细菌等微生物具有广谱抗性,且作用机制特殊。迄今为止,国内外在防御素方面进行了大量的研究,已经从各类生物体中分离出不同种类的防御素,并在基因工程和医药领域呈现广泛的应用前景。文章对防御素的分类、生物学特性,包括哺乳动物α-、β-、θ-防御素、昆虫以及植物防御素的分子结构及抗菌活性进行了综述,阐述了防御素的膜作用及与细胞内复合物结合的作用机制。总结和归纳了防御素基因的分离、表达研究进展及动、植物防御素基因在抗病基因工程领域的应用,并对防御素在未来的生物制药和植物抗病基因工程方面的应用前景进行了展望。Defensin is a kind of cysteine-rich small peptide,which has a broad spectrum of resistance to bacteria with a special resistance mechanism.So far,a large number of studies on defensins have been reported,and the different types of defensins have been isolated from various organisms.A broad prospect of application on defensins has been displayed both in genetic engineering and medicine field.This article reviewed the classification and the biological characteristics of defensins,including mammalian α-,β-,θ-defensins,insect defensins,and plant defensins.The molecular structures,antibacterial activities,and antibacterial mechanisms of these definsins were summarized.The two mechanisms of de-fensin,including independent membrane mechanism and targeting of intracellular compounds by defensins,are ex-pounded.This paper also summarized the researches on isolation and expression of defensin genes and disease resistance genetic engineering of mammal and plant defensins.A prospect of the future applications of defensin both in biophar-maceutical sciences and plant disease resistance genetic engineering was discussed.

关 键 词：防御素 抗病 基因工程 

分 类 号：Q516[生物学—生物化学]