海洋热带假丝酵母菌株中角鲨烯的分析与鉴定  

Analysis and Identification of Squalene from Marine Strain of Candida Tropicalis

作　　者：雷富[1] 何碧娟[1] 姜发军[1] 郑娟梅[1,2] 许铭本[1] 张荣灿[1] 

机构地区：[1]广西科学院,广西南宁530007 [2]广西中医学院,广西南宁530001

出　　处：《广西科学》2011年第2期161-163,共3页Guangxi Sciences

基　　金：广西科学院基本科研业务费项目(编号:09YJ17HY01)资助

摘　　要：从红树林白骨壤(Avicennia reacemosa)生长的高潮带土壤中分离得到1株热带假丝酵母(Candidatropicalis),用YPD培养基培养48h,离心收集菌体细胞,用正己烷萃取菌体中的脂溶性成分并用傅里叶红外光谱仪和气相色谱-质谱仪对其进行检测分析。结果表明菌株脂溶性成分傅里叶红外光谱与数据库中角鲨烯的红外光谱匹配度达到了91%。菌株脂溶性成分气相色谱-质谱与数据库中角鲨烯的质谱匹配度达99%,显著高于角鲨烯标准品的匹配度(95%)。热带假丝酵母细胞的角鲨烯最高含量达2.38mg/g(w/dw),表明该菌株中富含角鲨烯这种生物活性物质。A strain named Candida tropicalis,isolated from the soil of the mangrove Avicennia growth area in the high-tide of marina,was cultured in YPD medium for 48h.The yeast cells were extracted with hexane.The extract was analyzed by Fourier transform infrared spectroscopy and gas-mass chromatography.The results showed that the matching of fat-soluble component isolated from the strain with the squalene spectral library was 91% analyzed by Fourier transform infrared spectroscopy.The matching of fat-soluble component isolated from the strain with the squalene spectral library was 99% analyzed by GC-MS,which was significantly higher than the matching of standard squalene samples.The strain cells（dry weight） had 2.38 mg/g of squalene analyzed by mass spectrometry.This strain was rich in squalene marine active substance.

关 键 词：热带假丝酵母 海洋菌株 角鲨烯 

分 类 号：TQ95[化学工程]