分光光度法测定蒙药河柏中总酚酸的含量  

DETERMINATION OF TOTAL PHENOLIC ACIDS IN MYRICARIA ALOPCUROIDES SCHRENK BY SPECTROPHOTOMETRY

作　　者：薛培凤[1] 李占军[1] 解红霞[1] 王国英[1] 孟兆文[1] 程鹏[1] 

机构地区：[1]内蒙古医学院药学院,内蒙古呼和浩特010059

出　　处：《内蒙古医学院学报》2011年第1期43-45,53,共4页Acta Academiae Medicinae Neimongol

基　　金：内蒙古政府蒙药现代化重大研究专项蒙药材质量标准化资助项目(MX2005ZB012);内蒙古自治区高等学校科学研究项目(NJzy08098)

摘　　要：目的:建立测定河柏中总酚酸的含量的方法。方法:采用紫外可见分光光度法,检测波长720nm。结果:蒙药河柏中总酚酸在0.3088～1.0808μg/mL线性关系良好,回归方程为Y=0.6173X+0.0151(r=0.9998)。没食子酸的平均回收率为96.22%,RSD=1.13%(n=5)。结论:以紫外可见分光光度法测定河柏中总酚酸的含量,简便、灵敏、准确。Objective： To establish the determination method for total phenolic acid in Myricaria alopcuroides Schrenk.Methods：Using spectrophotometric determination with the detection wavelength of 720nm.Results：Samples of the total tration of phenolic acids 0.3088~1.0808μg/mL within linear relationship was found.The regression equation was Y=0.6173X＋0.0151（r=0.9998）.The average recovery for gallic acid was 96.22%（RSD = 1.13%）.Conclusion：The method is simple,sensitive,concenaccurate and can be used for determination of total phenolic acids in Myricaria alopcuroides Schrenk.

关 键 词：蒙药 河柏 总酚酸 没食子酸 分光光度法 

分 类 号：R927.2[医药卫生—药学]