ERK信号转导通路对新生大鼠海马神经干细胞增殖的影响  

Effect of ERK pathway on the proliferation of neural stem cells of hippocampus in neonatal rats

作　　者：佟雷[1] 王振宇[1] 季丽莉[1] 佟晓杰[1] 

机构地区：[1]中国医科大学基础医学院解剖学教研室,辽宁沈阳110001

出　　处：《解剖科学进展》2011年第3期232-235,共4页Progress of Anatomical Sciences

基　　金：高等学校博士学科点专项科研基金(20070159016);辽宁省自然科学基金(20082097)资助项目

摘　　要：目的探讨细胞外信号调解激酶(ERK)信号转导通路在新生大鼠海马神经干细胞增殖中的作用。方法体外培养新生大鼠海马神经干细胞,传至第4代后进行单细胞克隆培养并传代。将培养细胞分为2组,对照组采用神经干细胞培养液进行培养,实验组中在神经干细胞培养液的基础上添加不同浓度的ERK通路抑制剂U0126,Western Blot检测加入细胞中ERK及P-ERK的表达情况,MTT法检测其对神经干细胞增殖的影响。结果神经干细胞加入U0126后ERK磷酸化水平明显低于对照组;当U0126浓度为10M时,细胞的增殖速度较对照组明显降低。结论神经干细胞的增殖可能与ERK信号转导通路有关。Objective To study the effect of extracellular regulated signal kinase （ERK） pathway on the proliferation of neural stem cells of hippocampus in neonatal rats. Methods The hippoeampus of neonatal rat was removed sterilely, neural stem cells （NSCs） were collected and cultured in serum-free medium. The Cell suspension was prepared and single cell clone was performed when the diameter of the fourth passage of the clone sphere was 200 p. m, the monoclonal ceils were passaged and differentiated. NSCs were divided into control group cultured in the NSCs media and experimental group cultured in the mixture of NSCs media and ERK pathway inhibitor （U0126） . The expression of ERK protein was detected by western blot and the proliferation of NSCs was evaluated by MTT. Results The expression of phosphorylate ERK protein in NSCs was lower in the experimental group than control group, the proliferation rate of the NSCs was much lower in the experimental group than control group when the concentration of U0126 reached 10M. Conclusions The proliferation of neural stem cells decreased significantly when ERK signal transduction pathway was inhibited.

关 键 词：神经干细胞 增殖 细胞外信号调解激酶 新生大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]