碱性成纤维细胞生长因子对AD细胞模型ERK1/2活性的影响  

Effect of basic fibroblast growth factor on extracellular regulated protein kinase activity in Alzheimer's disease cell model

作　　者：吴哲[1] 赵艳华[2] 彭博[3] 罗晓光[1] 

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [2]阜新市中心医院神经内科 [3]中国医科大学门诊部内科

出　　处：《解剖科学进展》2011年第3期300-303,共4页Progress of Anatomical Sciences

基　　金：辽宁省博士启动课题(No.20051035)

摘　　要：目的研究碱性成纤维细胞生长因子(bFGF)对阿尔茨海默病(AD)细胞模型中磷酸化细胞外调节蛋白激酶(p-ERK1/2)表达影响。方法经体外培养的大鼠肾上腺嗜铬瘤细胞(PC12细胞)分正常对照组(常规培养液)、Aβ损伤组(即AD细胞模型,加入Aβ)、bFGF组(加入bFGF及Aβ)和抑制剂组(加25-3525-35入MEK抑制剂PD98059、bFGF和老化Aβ)。MTT法检测不同处理组PC12细胞存活率,WesternBlot免疫印迹25-35法分析p-ERK1/2蛋白表达变化。结果 Aβ损伤组PC12细胞存活率低于正常对照组(P<0.05),bFGF组细胞存活率高于Aβ损伤组(P<0.01)。WesternBlot结果显示,Aβ损伤组p-ERK1/2相对表达值较对照组显著下降,Aβ损伤组在加入不同浓度的bFGF后p-ERK1/2的相对表达值较Aβ损伤组显著增强,抑制剂组在加入不同浓度的PD98059后p-ERK1/2的相对表达值较bFGF组p-ERK1/2显著下降。结论 bFGF对Aβ诱导的PC12细胞损伤具有一定保护作用,可能与增强PC12细胞ERK1/2活性有关。Objective To study the effect of basic fibroblast growth factor （bFGF） on phosphorylate- extracellular regulated protein kinase （p-ERK1/2） in cell model of Alzheimer＇s disease （AD）. Methods Cultural rat pheochromocytoma cells （PC 12 ceils） were divided into 4 groups： normal control group （added cuhrue fluid）; A β damaged group （added cuhrue fluid and aged A β 25-35）; bFGF group （added cultrue fluid, bFGF and aged A β 25-35）; inhibitor group （added cultrue fluid, MEK inhibitor PD98059, bFGF and aged A β 25-35）. The cell survival rate of every group was detected by MTT, Western blot was used to determine p-ERK1/2 protein expression. Results Cell survive rate in A βdamaged group is significantly lower than that in the control （P〈O.05）, and cell survive rate of bFGF group is significantly higher than that in the A 13 damaged group （P〈O.O1）. Western blot results demonstrated that the expression level of p-ERK1/2 in A 13 damaged group was singnificantly lower than that in the control, higher in different concentrations of bFGF group than in A β damaged group with dose depentdent manner, lower in different concentrations of inhibitor group than in bFGF groups with dose dependent manner. Conclusions bFGF had protection on PC12 cells induced by A β , which might be related to enhancing activities of p-ERK.

关 键 词：碱性成纤维细胞生长因子 PC12细胞 Β-淀粉样蛋白 细胞外调节蛋白激酶 阿尔茨海默病 

分 类 号：R741[医药卫生—神经病学与精神病学]