Mouse cloning and somatic cell reprogramming using electrofused blastomeres  

作　　者：Amjad Riaz Xiaoyang Zha Xiangpeng Dai Wei Li Lei Liu Haifeng Wan Yang Yu Liu Wang Qi Zhou 

机构地区：[1]State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1st Beichen West Road, Beijing 100101, China [2]Graduate School, Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Cell Research》2011年第5期770-778,共9页细胞研究（英文版）

摘　　要：Mouse cloning from fertilized eggs can assist development of approaches for the production of ＂genetically tailored＂ human embryonic stem （ES） cell lines that are not constrained by the limitations of oocyte availability. However, to date only zygotes have been successfully used as recipients of nuclei from terminally differentiated somatic cell donors leading to ES cell lines. In fertility clinics, embryos of advanced embryonic stages are usually stored for future use, but their ability to support the derivation of ES cell lines via somatic nuclear transfer has not yet been proved. Here, we report that two-cell stage electrofused mouse embryos, arrested in mitosis, can support developmental reprogramming of nuclei from donor cells ranging from blastomeres to somatic cells. Live, full-term cloned pups from embryonic donors, as well as pluripotent ES cell lines from embryonic or somatic donors, were successfully generated from these reconstructed embryos. Advanced stage pre-implantation embryos were unable to develop normally to term after electrofusion and transfer of a somatic cell nucleus, indicating that discarded pre-implantation human embryos could be an important resource for research that minimizes the ethical concerns for human therapeutic cloning. Our approach provides an attractive and practical alternative to therapeutic cloning using donated oocytes for the generation of patient-specific human ES cell lines.

关 键 词：developmental reprogramming cleaved embryos nuclear transfer mitotic arrest ELECTROFUSION MG-132 

分 类 号：Q813[生物学—生物工程] S857.21[农业科学—临床兽医学]