ELECTROFUSION

作品数:18被引量:52H指数:3
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相关领域:生物学农业科学更多>>
相关作者:邓秀新刘继红胡春根周长河郭文武更多>>
相关机构:华中农业大学浙江大学更多>>
相关期刊:《Cellular & Molecular Immunology》《Science China(Life Sciences)》《Science China Chemistry》《Developmental and Reproductive Biology》更多>>
相关基金:国家自然科学基金国家重点基础研究发展计划教育部留学回国人员科研启动基金广东省自然科学基金更多>>
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Plant regeneration via protoplast electrofusion in cassava被引量:3
《Journal of Integrative Agriculture》2020年第3期632-642,共11页WEN Feng SU Wen-pan ZHENG Hua YU Ben-chi MA Zeng-feng ZHANG Peng GUO Wen-wu 
financially supported by the National Natural Science Foundation of China(31401438);the Innovation Research Team of the Ministry of Education of China(IRT_17R45);the earmarked fund for China Agriculture Research System(CARS-11-GXLJ);the Guangxi Scientific and Technological Development Subject,China(AB16380080 and AB16380163)
Protoplast electrofusion between callus protoplasts of cultivar TMS60444 and mesophyll protoplasts of cultivar SC8 was performed as an approach for the genetic improvement of cassava.The fusion products were subsequen...
关键词:tissue culture chromosome counting DNA loss ploidy analysis 
Effects of Polysilane-Coating on Interface of Electrofusion Joints for Maintaining Strength
《Materials Sciences and Applications》2015年第4期322-331,共10页Hiroaki Murase Shinichi Kawasaki Toshimichi Kitaoka Jouji Furukawa Hirofumi Ueda Hiroyuki Nishimura Kazushi Yamada 
The fusion strength of electrofusion joints using the polyethylene (PE) pipe connection greatly depends on the amount of sand which adheres to the interface by wind and so on, because there is no flow of melted resin ...
关键词:POLYSILANE ELECTROFUSION JOINT 
Generation and Developmental Characteristics of Porcine Tetraploid Embryos and Tetraploid/diploid Chimeric Embryos
《Genomics, Proteomics & Bioinformatics》2013年第5期327-333,共7页Wenteng He Qingran Kong Yongqian Shi Bingteng Xie Mingxia Jiao Tianqing Huang Shimeng Guo Kui Hu Zhonghua Liu 
supported by grants from the National Basic Research Program of China(973 Program;Grant No.2009CB941000 and 2011CB944202);the Fund for Outstanding Young Scholars in Heilongjiang Province(Grant No.JC200905)
The aim of this study was to optimize electrofusion conditions for generating porcine tet- raploid (4n) embryos and produce tetraploid/diploid (4n/2n) chimeric embryos. Different electric field intensities were te...
关键词:ELECTROFUSION TETRAPLOID CHIMERA Pig 
Improvement of chromium biosorption through protoplast electrofusion between Candida tropicalis and Candida lipolytica被引量:3
《Journal of Central South University》2012年第6期1693-1701,共9页何宝燕 尹华 杨峰 叶锦韶 彭辉 卢显妍 张娜 
Project(NSFC-GDNSF U0933002) supported by the Joint Funds of the National Natural Science Foundation of China and the Natural Science Foundation of Guangdong Province,China;Project(50978122) supported by the National Natural Science Foundation of China
Protoplasts from Candida tropicalis and Candida lipolytica were fused under an optimized electrofusion (electrical pulse strength 6 kV/cm, pulse duration time 40μs and pulse times 5) and then regenerated on YEPD me...
关键词:CHROMIUM BIOSORPTION FUSANT PROTOPLAST ELECTROFUSION 
Targeted Macromolecules Delivery by Large Lipidic Nanovesicles Electrofusion with Mammalian Cells
《Journal of Biomaterials and Nanobiotechnology》2011年第5期527-532,共6页Demange Pascal Réat Valérie Weinandy Stefan Ospital Remy Chopinet-Mayeux Louise Henri Pauline Milon Alain Teissié Justin 
Lipidic nanovesicles (so called liposomes) were one the earliest forms of nanovectors. One of their limits was our lack of knowledge on the delivery pathway of their content to the target cell cytoplasm. The present c...
关键词:ELECTROFUSION Delivery Systems in CANCER Liposomes Lipidic NANOVESICLES 
Mouse cloning and somatic cell reprogramming using electrofused blastomeres被引量:2
《Cell Research》2011年第5期770-778,共9页Amjad Riaz Xiaoyang Zha Xiangpeng Dai Wei Li Lei Liu Haifeng Wan Yang Yu Liu Wang Qi Zhou 
Mouse cloning from fertilized eggs can assist development of approaches for the production of "genetically tailored" human embryonic stem (ES) cell lines that are not constrained by the limitations of oocyte avail...
关键词:developmental reprogramming cleaved embryos nuclear transfer mitotic arrest ELECTROFUSION MG-132 
Electrofusion of tobacco protoplasts in space
《中国生物学文摘》2005年第7期98-98,共1页ZHENG Huiqiong WANG Liufa CHENG Aidi LIU Chengxian 
关键词:电镀溶解 烟草制品 原生质体 空间实验 实验研究 碳水化合物 地心引力 
Intergeneric Somatic Hybridization Between Brassica napus L. and Sinapis alba L.被引量:27
《Journal of Integrative Plant Biology》2005年第1期84-91,共8页You-PingWANG KarinSONNTAG EickeRUDLOFF Jian-MinCHEN 
教育部留学回国人员科研启动基金
: Electrically induced protoplast fusion was used to produce somatic hybrids between Brassica napus L. and Sinapis alba L. Seven hybrids were obtained and verified by the simple sequence repeat and cleaved amplified p...
关键词:Brassica napus L. ELECTROFUSION erucic acid intergeneric hybrid Sinapis alba L 
Specific Antitumor Effects of Tumor Vaccine Produced by Electrofusion between Osteosarcoma Cell and Dendritic Cell in Rats被引量:2
《Cellular & Molecular Immunology》2004年第6期454-460,共7页ZheYu QingyuFan XinbaoHao HuaLong 
the National Natural Science Foundation of China(No.30330610).
Dendritic cells (DCs) are potent antigen-presenting cells capable of inducing primary T-cell responses.Several immunotherapy treatment strategies involve manipulation of DCs,both in vivo and ex vivo,to promote the imm...
关键词:DC OSTEOSARCOMA TUMOR VACCINE IMMUNOTHERAPY 
Electrofusion of tobacco protoplasts in space被引量:2
《Chinese Science Bulletin》2003年第18期1967-1970,共3页Huiqiong Zheng Liufa Wang Aidi Cheng Chengxian Liu 
Electrofusion of evacuolated (N. Tabacum L. cvs. Gexin no1) and vacuolated (N. Rustic) tobacco mesophyll protoplasts was performed on the Chinese spacecraft (Shenzhou No. 4, from 30th Dec. 2002 to 6th Jan. 2003). The ...
关键词:microgravitation ELECTROFUSION VIABILITY YIELD of FUSION TOBACCO protoplast. 
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