financially supported by the National Natural Science Foundation of China(31401438);the Innovation Research Team of the Ministry of Education of China(IRT_17R45);the earmarked fund for China Agriculture Research System(CARS-11-GXLJ);the Guangxi Scientific and Technological Development Subject,China(AB16380080 and AB16380163)
Protoplast electrofusion between callus protoplasts of cultivar TMS60444 and mesophyll protoplasts of cultivar SC8 was performed as an approach for the genetic improvement of cassava.The fusion products were subsequen...
The fusion strength of electrofusion joints using the polyethylene (PE) pipe connection greatly depends on the amount of sand which adheres to the interface by wind and so on, because there is no flow of melted resin ...
supported by grants from the National Basic Research Program of China(973 Program;Grant No.2009CB941000 and 2011CB944202);the Fund for Outstanding Young Scholars in Heilongjiang Province(Grant No.JC200905)
The aim of this study was to optimize electrofusion conditions for generating porcine tet- raploid (4n) embryos and produce tetraploid/diploid (4n/2n) chimeric embryos. Different electric field intensities were te...
Project(NSFC-GDNSF U0933002) supported by the Joint Funds of the National Natural Science Foundation of China and the Natural Science Foundation of Guangdong Province,China;Project(50978122) supported by the National Natural Science Foundation of China
Protoplasts from Candida tropicalis and Candida lipolytica were fused under an optimized electrofusion (electrical pulse strength 6 kV/cm, pulse duration time 40μs and pulse times 5) and then regenerated on YEPD me...
Lipidic nanovesicles (so called liposomes) were one the earliest forms of nanovectors. One of their limits was our lack of knowledge on the delivery pathway of their content to the target cell cytoplasm. The present c...
Mouse cloning from fertilized eggs can assist development of approaches for the production of "genetically tailored" human embryonic stem (ES) cell lines that are not constrained by the limitations of oocyte avail...
: Electrically induced protoplast fusion was used to produce somatic hybrids between Brassica napus L. and Sinapis alba L. Seven hybrids were obtained and verified by the simple sequence repeat and cleaved amplified p...
the National Natural Science Foundation of China(No.30330610).
Dendritic cells (DCs) are potent antigen-presenting cells capable of inducing primary T-cell responses.Several immunotherapy treatment strategies involve manipulation of DCs,both in vivo and ex vivo,to promote the imm...
Electrofusion of evacuolated (N. Tabacum L. cvs. Gexin no1) and vacuolated (N. Rustic) tobacco mesophyll protoplasts was performed on the Chinese spacecraft (Shenzhou No. 4, from 30th Dec. 2002 to 6th Jan. 2003). The ...