Purification Technology and Antimicrobial Activity Analysis of Antimicrobial Peptide from Ovotransferrin  

作　　者：ZHANG Tie-hua ZHENG Jian YE Hai-qing YU Ya-li ZHAO Ping LIU Jing-bo 

机构地区：[1]College of Light Industry and Economics ＆ Management, Jilin University, Changchun 130062, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第3期361-365,共5页高等学校化学研究（英文版）

基　　金：Supported by the Applied Basic Fund of Jilin Provincial Science and Technology Department(No.20070572);the Frontiers of Science of Jilin University and Innovation Fund of Interdisciplinary, China(No.200903224)

摘　　要：Antibacterial peptides mixture purified from Ovotransferrin by pepsin digest was used as the raw material. Peptide sections with good antibacterial activity were determined after bacteriostasis experiments, its molecular weight and amino acid composition were analyzed. The results of experiments indicate that with Sephadex G-50 and distilled water as mobile phase, detection wavelength 220 nm, flow rate 1.5 mL/min, sample density 0.2 g/mL, and volume 0.2 mL are the optimal conditions. Bacteriostasis experiments of the fraction of purified peaks were carried out and the result was： peak 1peak 3peak 2; the molecular weight of peak 1 was about 3015 by high performance liquid chromatography; active peptide possessed positive charges by amino acid analysis, its cationic characteristics are in accordance with the nature of antimicrobial peptides.Antibacterial peptides mixture purified from Ovotransferrin by pepsin digest was used as the raw material. Peptide sections with good antibacterial activity were determined after bacteriostasis experiments, its molecular weight and amino acid composition were analyzed. The results of experiments indicate that with Sephadex G-50 and distilled water as mobile phase, detection wavelength 220 nm, flow rate 1.5 mL/min, sample density 0.2 g/mL, and volume 0.2 mL are the optimal conditions. Bacteriostasis experiments of the fraction of purified peaks were carried out and the result was： peak 1peak 3peak 2; the molecular weight of peak 1 was about 3015 by high performance liquid chromatography; active peptide possessed positive charges by amino acid analysis, its cationic characteristics are in accordance with the nature of antimicrobial peptides.

关 键 词：OVOTRANSFERRIN PURIFICATION Molecular weight Amino acid 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程] Q516[轻工技术与工程—食品科学与工程]