Intestinal Transport and Biotransformation of Resibufogenin and Cinobufagin in Chan Su via HPLC/APCI-MS^n  

作　　者：HAN Tian-jiao WANG Qing SONG Feng-rui LIU Zhong-ying LIU Zhi-qiang LIU Shu-ying 

机构地区：[1]Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun Center of Mass Spectrometry,Changchun 130022,P.R.China [2]Department of Endocrinology of China-Japan Union Hospital of Jilin University,Changchun 130033,P.R.China [3]School of Pharmacy,Jilin University,Changchun 130033,P.R.China [4]Graduate School of the Chinese Academy of Sciences,Beijing 100049,P.R.China

出　　处：《Chemical Research in Chinese Universities》2011年第3期380-384,共5页高等学校化学研究(英文版)

基　　金：Supported by the National Natural Science Foundation of China(Nos.30772721 and 30873360)

摘　　要：In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MSn. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability coefficients(Papp) of resibufogenin and cinobufagin are higher than 10–6 cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetylcinobufagin and 3-epideacetycinobufagin, respectively.In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MSn. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability coefficients(Papp) of resibufogenin and cinobufagin are higher than 10–6 cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetylcinobufagin and 3-epideacetycinobufagin, respectively.

关 键 词：Chan Su RESIBUFOGENIN CINOBUFAGIN Caco-2 cell monolayer Human intestinal bacteria 

分 类 号：R285[医药卫生—中药学]