Toxicity of Naphthalene on Caenorhabditis elegans  

作　　者：ZHANG Shu-hua XU Jing-bo LIU Cheng-bai LI Qiao GUAN Shu-wen WANG Le-ping 

机构地区：[1]College of Urban and Environmental Science, Northeast Normal University, Changchun 130024, R R. Chinal [2]Life Science and Technology Institute, Changchun University of Science and Technology, Changchun 130022, P. R. China,＂ [3]Life Science College, Jilin University, Changchun 130012, P. R. China [4]China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第3期468-473,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.20877017);the National High Technology Research and Development Program of China (No.2007AA03Z314)

摘　　要：Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular-level effect, stress-related gene expression was investigated such as those of hsp-16.1, sod-3, ctl-2, cep-1, cyp35a2, ugt-44, gst-1 and dhs-28. Cell apoptosis was assessed at the biochemical level. Life span, locomotion behaviors and brood size were investigated at the physiological level. The results indicate that naphthalene exposure could not only induce the expression of stress-related genes such as hsp16.1, sod-3, ctl-2 and cep-1 but also reduce the life span of Caenorhabditis elegans. At the same time, naphthalene exposure could result in cell apoptosis and interfere in the locomotion behaviors of Caenorhabditis elegans. These data suggest that naphthalene has multiple toxicity on Caenorhabditis elegans.Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular-level effect, stress-related gene expression was investigated such as those of hsp-16.1, sod-3, ctl-2, cep-1, cyp35a2, ugt-44, gst-1 and dhs-28. Cell apoptosis was assessed at the biochemical level. Life span, locomotion behaviors and brood size were investigated at the physiological level. The results indicate that naphthalene exposure could not only induce the expression of stress-related genes such as hsp16.1, sod-3, ctl-2 and cep-1 but also reduce the life span of Caenorhabditis elegans. At the same time, naphthalene exposure could result in cell apoptosis and interfere in the locomotion behaviors of Caenorhabditis elegans. These data suggest that naphthalene has multiple toxicity on Caenorhabditis elegans.

关 键 词：NAPHTHALENE Caenorhabditis elegans TOXICITY Real-time polymerase chain reaction（RT-PCR） 

分 类 号：Q959.17[生物学—动物学] TQ241.52[化学工程—有机化工]