HBV和AFB_1引起树鼩肝癌形成过程中IGF-Ⅱ蛋白表达的动态观察  

EXPRESSION OF INSULIN LIKE GROWTH FACTOR Ⅱ (IGF Ⅱ) PROTEIN DURING TREE SHREWS' HEPATOCARCINOGENESIS DIFFERENTLY INDUCED BY AFB 1 AND/OR HBV

作　　者：李瑗 苏建家 严瑞琪[2] 覃柳亮 杨春 班克臣 段小娴 黄国华 

机构地区：[1]广西肿瘤研究所病理研究室,南宁530021 [2]中山医科大学肿瘤研究所病理研究室,广州51006

出　　处：《广西医科大学学报》1999年第4期395-398,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金!项目 ( 3 92 6 0 0 3 3 )资助

摘　　要：目的 :观察不同病因如黄曲霉毒素 B1 ( AFB1 )和 (或 )人乙型肝炎病毒 ( HBV )诱发树肝细胞癌 ( HCC)形成过程中 ,胰岛素样生长因子 ( IGF- )的动态变化。方法 :成年树按不同处理分为 A ( HBV+AFB1 )、 B ( HBV)、 C( AFB1 )、D (空白对照 ) 4组。各组动物定期肝活检 ;存活动物于实验 160周时处死。各次活检以及处死的动物肝组织均用免疫组化 S- P法进行 IGF- 蛋白表达的检测 ,并辅以常规病理组织学及 HBs Ag等 HBV感染标志的检查。结果 :HCC的发生仅见于 A、 C组 ,分别为 66.7% ( 14 / 2 1)和 3 0 .0 % ( 3 / 10 ) ,该两组的 IGF- 阳性率曲线亦高于其余两组 ,其中 C组的曲线虽曾在实验中期高于 A组 ,但不稳定 ;A组则于实验期间一直较稳定地保持在较高水平。B组仅有大增生结节但无 HCC发生 ,该组的 IGF- 阳性表达率曲线在 3个实验组中最低 ,仅高于空白对照 D组。结论 :IGF- 在 HCC的形成过程中呈较稳定、高水平的表达 ,但这种现象与Objective:To observe the expression of insulin like growth factor Ⅱ (IGF Ⅱ) during tree shrews' hepatocarcinogenesis induced differently by aflatoxin B 1 (AFB 1) and /or hepatitis B virus (HBV) Methods:Healthy adult tree shrew were treated differently in the four groups such as group A (HBV+AFB 1),B (HBV),C (AFB 1) and D (control) Liver biopsies from the animals in each groups were taken regularly At the end of the 160th week the survivals were all sacrificed All the liver tissues from biopsies and autopsies were detected for IGF Ⅱ protein immunohistochemically,histological changes and HBV markers were observed as well Results:Hepatocellular carcinoma (HCC) occurred only in group A and group C,with the incidence of 67% (14/21) and 30% (3/10) respectively The curve for the positive ratios of IGF Ⅱ expression at different time during the experiment in these two groups were also higher than the other two groups Between them,the curve for group C was once higher than that of group A,but the curve for group A were constantly keeping in the high lever since the middle of the experiment In group B,only large hepatocellular proliferating nodules but no HCC were observed The ratio of IGF Ⅱ positive expression in this group was also lower than those of group A and C,and only a little higher than that of the control group Conclusions:There is constantly high expression of IGF Ⅱ during tree shrews' hepatocarcinogenesis This abnormality,however,is not related to the inducing factors whether they are chemicals or virus

关 键 词：IGF-Ⅱ 肝细胞癌 树QU 乙型肝炎病毒 AFB1 

分 类 号：R735.702[医药卫生—肿瘤]