NF-kB拮抗剂PDTC对HepG2细胞的抑制及对caspase-3表达的影响  

Effect of NF-kB Antagonist PDTC in Inhibition of Human Hepatic Carcinoma Cell HepG2

作　　者：石炜[1] 曾斌[1] 张杰[1] 谈高[1] 熊丹[1] 

机构地区：[1]南华大学附属第一医院消化内科,湖南衡阳421001

出　　处：《现代生物医学进展》2011年第9期1651-1654,共4页Progress in Modern Biomedicine

摘　　要：目的:研究NF-kB拮抗剂PDTC诱导人肝癌HepG2细胞凋亡及其对凋亡相关基因caspase-3表达的影响,初步探讨其诱导凋亡的可能机制。方法:以不同浓度的PDTC处理人肝癌HepG2细胞,利用MTT法检测对人肝癌HepG2细胞凋亡的影响;利用RT-PCR和Western-blot检测caspase-3mRNA和蛋白的表达。结果:不同浓度PDTC作用人肝癌HepG2细胞不同时间后,能够显著抑制HepG2细胞的生长增殖,存在剂量和时间依赖性(P<0.05);PDTC能够上调HepG2细胞中caspase-3mRNA和蛋白的表达。结论:NF-kB拮抗剂PDTC对人肝癌HepG2细胞产生显著抑制,并能上调HepG2细胞中caspase-3mRNA和蛋白的表达。Objective： To investigate the role of apoptosis by PDTC,the selective inhibit of NF-kB on human hepatic carcinoma cell （HepG2）. and the effect of expression of csaspase-3,principium disguisition the likely mechalism which induce HepG2 cells apoptosis. Methods： After treating human hepatic carcinoma cell （HepG2） by PDTC, MTT assay was used to observe the growth inhibition of cell- HepG2. The mRNA and protein expression of caspase-3 were detected by RT-PCR and Western-blot assay, respectively. Results： When PDTC was given, growth inhibition and elevated apoptosis of HepG2 cells were detected,which was showed in a dose- and time- - depen- dent manner, PDTC up-regulated the lever of mRNA and protein expression of caspase-3. Conclusion： PDTC can induce apoptosis of human hepatic carcinoma cell HepG2 cells,which may be performed by up-regulated of caspase-3 expression.

关 键 词：PDTC 人肝癌HEPG2细胞 CASPASE-3 

分 类 号：R735.7[医药卫生—肿瘤]