直接注射法制作ICR小鼠肝癌原位移植瘤模型  

Making an Orthotopic Tumor Model of Hepatocellular Carcinoma for ICR Mice by Direct Injection

作　　者：张建民[1] 施晓雷[2] 陶科[1] 潘军平[1] 吴亚夫[2] 

机构地区：[1]南京医科大学鼓楼临床医学院肝胆外科,江苏南京210008 [2]南京医科大学附属鼓楼医院肝胆外科,江苏南京210008

出　　处：《现代生物医学进展》2011年第9期1680-1682,共3页Progress in Modern Biomedicine

基　　金：南京市卫生局医学科技发展项目(ZKX09027)

摘　　要：目的:培养鼠肝癌H22细胞,直接注射法制作ICR小鼠肝癌原位移植瘤,为后续实验奠定基础。方法:鼠肝癌H22细胞体外培养,将调整好的对数生长期的肝癌细胞直接注射小鼠肝脏,2周后解剖观察,并进行组织HE染色。结果:所有实验小鼠均可见肿瘤生长,HE染色示肝细胞肝癌。结论:直接注射法制作ICR小鼠肝癌原位移植瘤模型简便易行,值得推广应用。Objective： To cultivate H22 cell line and making an orthotopic tumor model of hepatocellular carcinoma on ICR mouse using direct injection method to lay the foundation for subsequent experiment. Methods： Cultivate H22 cell line in vitro and an orthotopic xenograft tumor model of hepatocellular carcinoma was created by injection of H22 cells in logarithmic growth phase directly into the liver parenchyma of ICR mice. Two weeks later, hepatocellular carcinoma specimens of animals were observed and stained with hematoxylin/eosin. Results： Tumor growth happened on all the experimental mice and was showed as hepatocellular carcinoma by hematoxylin-eosin staining. Conclusion： It is convenient of direct injection method on making an orthotopic tumor model of hepatocellular carcinoma and should be reported and used widely.

关 键 词：ICR小鼠 癌 肝细胞 模型 动物 

分 类 号：Q95-3[生物学—动物学] R735.7[医药卫生—肿瘤]