脑胶质瘤中CDK_4基因扩增与p16基因缺失的相关性研究  

CDK\-4 gene amplification and p16 gene deletion in human glioma

作　　者：田新华[1,3] 江澄川[1] 许凯黎[2] 周瑾[2] 高翔[1] 陈商群[1] 

机构地区：[1]上海华山医院神经外科,200040 [2]上海市肿瘤研究所 [3]山西医科大学附属第二医院

出　　处：《中华实验外科杂志》1999年第5期393-394,共2页Chinese Journal of Experimental Surgery

基　　金：国家教委博士点基金

摘　　要：目的　探讨ｐ１６ 基因、 Ｃ Ｄ Ｋ４ 基因异常与脑胶质瘤发生发展的相关性。方法　应用 Ｐ Ｃ Ｒ分子杂交技术（ Ｓｏｕｔｈｅｒｎ Ｂｌｏｔ 和 Ｓｌｏｔ Ｂｌｏｔ） 检测４３ 例不同病理分级的脑胶质瘤中 Ｃ Ｄ Ｋ４ 基因扩增与ｐ１６基因缺失情况。结果　ｐ１６ 基因在Ⅰ～Ⅱ、Ⅲ级、Ⅳ级肿瘤中的缺失率分别为９ ％ （１／１１） 、２９ ％ （５／１７） 、４０ ％ （６／１５） ； Ｃ Ｄ Ｋ４ 基因扩增主要出现于Ⅲ级肿瘤（２５ ％ ） ，频发于Ⅳ级肿瘤（５５ ％ ） ，扩增状况与恶性程度呈正相关。结论　 Ｃ Ｄ Ｋ４ 基因扩增、ｐ１６ 基因缺失的细胞周期监控失调与脑胶质瘤发生发展关系密切， Ｃ Ｄ Ｋ４ 基因作用的强化与ｐ１６ 基因的功能失活，均可促发脑胶质细胞的癌性增殖或脑胶质瘤细胞的恶性演进。Objective\ To investigate CDK4 gene amplification or p16 gene deletion in human gliomas and relationship between the changes of p16, CDK 4 gene and the clinical pathology.Methods\ CDK 4 gene amplification and p16 gene deleton in cases of brain gliomas with different pathologic types were detected by using PCR, Southern blot an Slot blot.Results\ The deletion rate of p16 gene was 9% (1/11) of grade Ⅰ Ⅱ, 29%(5/17) of grade Ⅲ, 40%(6/15) of grade Ⅳ. The amplification of CDK 4 gene primarily occurred in grade Ⅲ (25%), frequently occurred in grade Ⅳ (55%). Positive correlation was found between the rate of amplification and the degree of malignancy.Conclusion\ The suppression of p16 gene or the increased activity of CDK 4 gene were sufficient to disrupt the cell cycle regulatory mechanism in a progression.\;

关 键 词：脑胶质瘤 P16基因 CDK4基因 基因缺失 

分 类 号：R739.410.2[医药卫生—肿瘤]