丛粒藻形态多样性与遗传多样性研究  

Morphological and Genetic Diversity of Botryococcus braunii

作　　者：王朋云[1] 茅云翔[1] 孔凡娜[1] 马梅[1] 马飞[1] 

机构地区：[1]中国海洋大学海洋生物遗传学与基因资源利用教育部重点实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2011年第5期63-70,共8页Periodical of Ocean University of China

基　　金：国家重点基础研究发展计划项目(2011CB200901)资助

摘　　要：观察了采集自不同地点的3株丛粒藻(Botryococcus braunii)(AGB-Bb01、AGB-Bb02和AGB-Bb03)的显微结构和亚显微结构,以18S rDNA和ITS区序列为目标位点比较分析了16株丛粒藻藻株的遗传距离和序列相似性,重建了系统发生树。结果表明:丛粒藻不同地理株在细胞大小、聚落大小和聚落细胞数目方面存在较明显的差异,亚显微结构显示不同藻株的杯状鞘厚度及细胞包埋程度也存在差异;不同地理株间具有较高的遗传多样性,系统发生树显示所有藻株可分成2个簇群和4个亚群,存在一定程度的地理隔离。研究证明了18S rDNA和ITS区序列是进行丛粒藻基因分型和遗传多样性研究的良好位点。本研究为系统了解丛粒藻的遗传多样性和开展优良藻株选育工作奠定了基础。We observed the microscopic structure and sub-microscopic structure of three Botryococcus braunii strains（AGB-Bb01, AGB-Bb02 and AGB-Bb03） isolated from different sites. Also, the phyloge- netie trees involved in 16 strains of B. braunii were reconstructed based on the genetic distances and se- quence similarities of 18S rDNA and ITS region sequences. The results showed that the cell size, colony size and cell number in one colony of the three geographical strains were significantly different. The sub- microscopic structures demonstrated that there were difference on the thickness of the cup-sheath and ex- tent of cell embedded by the cup-sheath among the three strains as well. There existed high genetic diversity among the different strains living in the different sites and phylogenetic tree showed that all the strains can be divided into 2 groups and 4 sub-groups showing the geographical isolation. It is proved that sequences of 13S rDNA and ITS region can be considered as good tools for gene-type identification and ge- netic diversity research in B. braunii. The study lays a solid foundation for the research of the genetic diversity and cultivation in B. braunii.

关 键 词：丛粒藻 亚显微结构 18S-28S rDNA形态多样性 遗传多样性 

分 类 号：Q344[生物学—遗传学]