体外培养的男性外生殖器皮肤成纤维细胞单层5α-还原酶活性测定  

Measurement of 5α reductase activity with cultured male genital skin fibroblast monolayer

作　　者：伍学焱[1] 史轶蘩[2] 邓洁英[3] 

机构地区：[1]中国医学科学院,100730 [2]中国协和医科大学,100730 [3]北京协和医院内分泌科,100730

出　　处：《中华内分泌代谢杂志》1999年第4期216-219,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的　提高临床对两性畸形患者的诊断和治疗水平。方法　以体外培养的男性外生殖器皮肤成纤维细胞单层为５α还原酶活性测定材料，３ Ｈ睾酮（ Ｔ） 为底物，经薄层层析法分离产物３ Ｈ去氢睾酮（ Ｄ Ｈ Ｔ） ，然后，结合蛋白质含量计算５α还原酶活性。结果　建立了稳定可靠的５α还原酶测定方法。批内和批间变异系数分别为６ ．４ ％ 和８ ．９ ％ 。总回收率为９１ ．５ ％ 。８ 例正常人５α还原酶活性为（７ ．００ ±９ ．４１）ｐｍ ｏｌ·ｍｇ － １·ｈ － １ （１ ．０８ ～２３ ．２１ｐ ｍｏｌ·ｍ ｇ － １ ·ｈ － １ ） 。结论　正常人之间５α还原酶活性存在较大变异。Objective To improve the diagnosis and treatment of sex differentiation disorder. Methods The 5α reductase activity measurement was established with the intact genital skin fibroblast monolayer. Results The inter and intra coefficients of variability were 6.4% and 8.9% respectively. After extraction and chromatography, the total recovery rate of 3H DHT was 91.5%. The value of 5α reductase activity in 8 normal males were (7.00±9.41)pmol·mg -1 ·h -1 (1.08～23.21pmol·mg -1 ·h -1 ). Conclusion The method of 5α reductase activity mesurement was stable and an evident variability was revealed among normal males.

关 键 词：假两性畸形 5Α-还原酶 诊断 GSF 

分 类 号：R586.904[医药卫生—内分泌]