低频超声辐照联合微泡抑制兔VX_2肿瘤生长的实验研究  

An experiment study on the inhibition of rabbit VX_2 soft-tissue tumor by low frequency ultrasound radiation and microbubbles

作　　者：王宇[1] 申锷[2] 胡兵[1] 

机构地区：[1]上海交通大学附属第六人民医院超声医学科 [2]上海超声医学研究所,上海200233

出　　处：《现代肿瘤医学》2011年第6期1063-1066,共4页Journal of Modern Oncology

基　　金：上海市科委基础研究重点项目(编号:10JC14125600)

摘　　要：目的:探索低频超声辐照联合静脉注射微泡抑制肿瘤生长的非创伤性治疗肿瘤的新方法。方法:24只新西兰大白兔后腿肌肉内接种VX2肿瘤,瘤体长至1cm左右随机分为对照组、单纯微泡组、单纯超声组和超声微泡组。各组经相应处理后,超声检测肿瘤大小、观察瘤体内血流灌注情况,绘制肿瘤生长曲线,计算肿瘤增长百分率。病理学观察治疗后各组瘤体组织的病理学损伤。结果:治疗后2周结果显示超声微泡组瘤体体积和肿瘤增长百分率均明显小于其余各组(P<0.01),瘤体内血流灌注明显减少,瘤体组织大部分坏死;单纯微泡组、单纯超声组的瘤体体积和肿瘤增长百分率与对照组间均无显著性差异(P>0.05)。结论:20kHz超声辐照联合微泡造影剂SonoVue可有效抑制兔VX2肿瘤的生长。Objective：To explore a noinvasive therapy of tumor by cavitation effect induced by microbubbles mediated low frequency ultrasound radiation.Methods： VX2 tumor was implanted into the unilateral proximal thigh of 24 Newzealand white rabbits successfully.These rabbits were randomly divided into four groups,control group,simplicity microbubble group,simplicity ultrasound group,and ultrasound and microbubble group,when the tumors approached about 1.0 centimeter in size,with 6 rabbits in each group.After treatment correspondently,measured the volume of gross tumor and observed the blood flow infusion by sonography for 2 weeks.Then,drew a growth curve and calculated the ratio of tumor growth,collected the tumor samples and histopathology damages were observed.Results： After treatment for two weeks,tumor volume and tumor growth ratio of ultrasound and microbubble group were significantly smaller than the other groups（P0.01）,the blood flow reduction was observed by contrast enhanced ultrasonography.Necrosis was observed by histopathology.No statistical difference among control group,simplicity microbubble group and simplicity ultrasound group.Conclusion： Low frequency ultrasound radiation of 20kHz with microbubble sonoVue in vascular can inhibit the growth of VX2 soft-tissue tumors in rabbits.It might be the mechanism of the VX2 tumor microcirculation being selectively destroyed by microbubbles mediated ultrasound cavitation.

关 键 词：低频超声 超声空化 兔VX2肿瘤 微泡 

分 类 号：R730.59[医药卫生—肿瘤]