结直肠癌组织MMP-7、MMP-9表达与腹腔微转移的相关性研究  

Relationship between the expressions of MMP-7,MMP-9 and the abdominal cavity metastasis in colorectal carcinoma

作　　者：马顺茂[1] 贾漪涛[2] 刘红磊[1] 杜芸[3] 王永军[3] 李中信[3] 

机构地区：[1]河北医科大学附属华北石油管理局总医院,河北任丘062552 [2]河北省人民医院肿瘤科,河北石家庄050051 [3]河北医科大学第四医院,河北石家庄050011

出　　处：《现代肿瘤医学》2011年第6期1157-1160,共4页Journal of Modern Oncology

基　　金：河北省卫生厅重点课题计划(No.07109)

摘　　要：目的:探讨MMP-7、MMP-9在结直肠癌腹腔微转移中的作用以及相关性。方法:收集98例结直肠癌患者手术中腹腔冲洗液,进行CEA、CK20免疫细胞化学染色确定腹腔微转移。使用组织阵列仪制作组织芯片,进行免疫组化染色(SP),检测MMP-7、MMP-9在结直肠癌组织中的表达情况,分析其与腹腔微转移的关系。结果:CEA、CK20联合检测腹腔微转移率为32.7%(32/98)。MMP-7、MMP-9在伴有腹腔微转移的结直肠癌中阳性表达率分别为93.75%和96.88%,明显高于无腹腔微转移结直肠癌表达率72.73%和68.18%(P<0.05,P<0.01)。结论:MMP-7、MMP-9可能在结直肠癌腹腔微转移的发生过程中起重要作用。Objective：To estimate the relationship between MMP-7,MMP-9 expression and the abdominal cavity metastasis in colorectal carcinoma.Methods： Total of 98 cases diagnosed as colorectal carcinoma were studied.The free cancer cells in abdominal cavity were detected by routine staining and CEA or CK20 immunostaining.The expression of MMP-7 and MMP-9 in colorectal carcinoma tissues were detected by tissue chip array and SP immunohistochemistry.Results： Free cancer cells were found in 32.7%（32/98） cases of colorectal cancer.The positive rates of MMP-7 and MMP-9 expressions were 93.75% and 96.88% respectively in colorectal cancer with abdominal cavity metastasis,which were higher than those without abdominal cavity metastasis（72.73% and 68.18%,respectively）（P0.05）.Conclusion： MMP-7 and MMP-9 may play important roles in the development of abdominal cavity metastasis in colorectal carcinoma.

关 键 词：结直肠癌 腹腔微转移 MMP-7 MMP-9 组织微阵列/组织芯片 

分 类 号：R735.33[医药卫生—肿瘤] R735.37[医药卫生—临床医学]