体外培养神经干细胞CNTF、TGF-β1和IGF-1的表达  

Expression of CNTF,TGF-β1,and IGF-1 in NSCs Cultured in vitro

作　　者：赵楠[1] 刘俊[1] 林娜[2] 王廷华[2] 

机构地区：[1]昆明市第一人民医院神经外科,云南昆明650031 [2]昆明医学院神经科学研究所,云南昆明650031

出　　处：《昆明医学院学报》2011年第3期12-15,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30260125);云南省自然科学基金资助项目(2008cd195-1)

摘　　要：目的研究培养神经干细胞(NSC)中CNTF,TGF-β1,IGF-1基因表达情况.方法取小鼠胎鼠海马,分离纯化培养NSC,提取总RNA,RT-PCR技术检测CNTF,TGF-β1,IGF mRNA的表达情况.结果培养NSC中各因子mRNA均有表达;CNTF,TGF-β1表达较强,IGF-1表达较弱.结论体外培养的NSC表达CNTF,TGF-β1含量较高,可能是应用其治疗神经疾病的理论基础.Objective To study the expression of neurotrphic factors（NTFs）,known as Ciliary Neurotrophic Factor（CNTF）,insulin-like growth factor-1（IGF-1）,transforming growth factor-beta1（TGF-β1）,in the neural stem cells（NSCs）of mice.Methods NSCs were isolated from hippocampus of mice embryo and cultured for 7 days,then were identified by nestin immunohistochemisstry method.Total RNA of NSCs was extracted to determine the expression of mRNA of IGF-1,CNTF,and TGF-β1 by RT-PCR,respectively.Results The mRNA exression for three growth factors were seen in cultured NSC.The expression of CNTF and TGF-β1 was more stronger than IGF-1expression.Conclusions NSCs cultured in vitro can express many neurotrphic factors,the expression levels of CNTF and TGF-β1 are high.This study provides crucial evidence on the application of NSCs in nervous system diseases.

关 键 词：小鼠神经干细胞 CNTF TGF-Β1 IGF-1 RT-PCR 

分 类 号：R741.05[医药卫生—神经病学与精神病学]