CK19和EGFR mRNA在喉癌动物模型外周血中的表达及其与淋巴转移关系  

Expression of CK19 mRNA and EGFR mRNA in the peripheral blood and its relationship with lymphatic metastasis in laryngeal carcinoma model

作　　者：吴蔚[1] 胡国华[1] 康苧心[1] 蒋建国[1] 杨旋[1] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉科重庆,400016

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第11期501-505,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨循环播散肿瘤细胞(CTC)与喉癌淋巴结转移的相关性,评价细胞角蛋白(CK19)和表皮生长因子受体(EGFR)在喉癌微转移诊断中的价值。方法:从30只裸鼠中随机选择25只均分为5组建立喉肿瘤模型,剩余5只为对照组,分别于接种后2、4、6、8、10周处死1组。采用RT-PCR方法检测裸小鼠外周血及肿瘤组织中CK19mRNA和EGFR mRNA的表达,采用连续病理切片苏木精-伊红染色观察淋巴结转移,免疫组织化学(SP)法检测肿瘤组织中EGFR的表达。结果:对照组外周血中未发现CK19mRNA和EGFR mRNA的表达,实验组外周血和肿瘤组织中CK19mRNA阳性率分别为48%和80%,EGFR mRNA的阳性率分别为36%和76%。淋巴结转移与外周血CTC有关(r=0.655,P<0.01),且多发生在肿瘤指数生长期,转移率为60%(15/25)。免疫组织化学检测EGFR蛋白在肿瘤组织阳性率为88%(22/25),外周血中EGFR mRNA表达阳性的肿瘤组织中EGFR蛋白均阳性,肿瘤组织EGFR蛋白高表达的外周血中EGFR mRNA检出率亦高。结论:RT-PCR检测外周血CK19和EGFR mRNA的表达有助于预测淋巴结转移,EGFR mRNA有望作为肿瘤诊断和治疗的新靶点。Objective：To explore clinical evaluate of CK19 mRNA and EGFR mRNA for diagnosis of laryngeal carcinoma micrometastasis,correlation between circulation tumor cell and lymph node metastasis.Method：Of 30 nude mice,25 were randomly divided into 5 experimental groups（5 mice in each group）,5 were acted as control group.The mice were killed 2,4,6,8 and 10 weeks after injection.The expression of CK19 and EGFR mRNA in the peripheral blood and tumor tissue were detected by RT-PCR assay.The expression of EGFR in tumor tissue were detected by immunohistochemistry and lymph node transfer were confirmed using continuous pathological dying.Result：None of CK19 and EGFR mRNA were detected in peripheral blood of control group,CK19 mRNA-positive rate was 48% and 80% in peripheral blood and tumor tissue from the experimental group,respectively,and EGFR mRNA-positive rate was 36% and 76%,respectively.Lymph node metastasis happened in the exponential growth phase and transfer rate was 60%（15/25）.The expression of CK19 mRNA and EGFR mRNA in lymphatic metastasis groups was higher than that of control,with a positive correlation between lymphatic metastasis and CTC（r=0.655,P〈0.01）.The protein positive expression rate of EGFR were 88%（22/25） in tumor tissue.All peripheral blood expressed EGFR concomitanted EGFR expressing in tumor tissues,and a high expression of EGFR in tumor tissue displayed high expression of EGFR in peripheral blood as well.Conclusion：The expression of CK19 and EGFR mRNA in the peripheral blood can provide predictive information of lymphatic metastasis,EGFR mRNA might be a new target of treatment and diagnosis for malignant tumour.

关 键 词：细胞角蛋白19 表皮生长因子受体 循环播散肿瘤细胞 喉肿瘤 裸鼠 微转移 淋巴转移 

分 类 号：R739.65[医药卫生—肿瘤]