疱疹病毒Ⅰ型胸苷激酶基因和更昔洛韦系统对恶性胶质瘤患者免疫功能的影响  

Effect of herpes simplex virus Ⅰ thymidine kinase gene and ganciclovir system on immunological functions in malignant glioma patients

作　　者：张晓鹏[1] 胡加飞[2] 李莉[2] 蔡如珏[2] 卢亦成[2] 朱诚[2] 

机构地区：[1]第二军医大学长征医院实验诊断科 [2]第二军医大学长征医院神经外科,上海200003

出　　处：《第二军医大学学报》1999年第9期611-613,共3页Academic Journal of Second Military Medical University

基　　金：国家"863"基金

摘　　要：目的：探讨疱疹病毒Ⅰ型胸苷激酶基因和更昔洛韦（ Ｈ Ｓ Ｖ Ｔ Ｋ／ Ｇ Ｃ Ｖ）系统对恶性胶质瘤患者体液免疫和细胞免疫功能的影响。方法：对１２ 例恶性胶质瘤患者采用 Ｈ Ｓ Ｖ Ｔ Ｋ／ Ｇ Ｃ Ｖ 试验治疗。分别于治疗前、治疗过程中和治疗后采外周血和脑脊液，用流式细胞仪检测外周血 Ｔ淋巴细胞亚群，用免疫比浊法测定血清和脑脊液免疫球蛋白的含量。结果：血 Ｃ Ｄ４＋ Ｔ 细胞在治疗开始后２ 周显著升高；自然杀伤（ Ｎ Ｋ）细胞在治疗过程中明显下降，治疗结束后则显著高于治疗前水平；细胞毒性细胞（ Ｃ Ｔ Ｌ）在治疗结束后显著高于治疗前水平；血清 Ｉｇ Ｍ 和脑脊液 Ｉｇ Ｇ、 Ｉｇ Ａ 和 Ｉｇ Ｍ 水平在治疗结束后均显著高于治疗前水平。结论： Ｈ Ｓ Ｖ Ｔ Ｋ／ Ｇ Ｃ Ｖ 系统对机体的体液和细胞免疫功能有明显的促进作用。Objective: To evaluate the effect of herpes simplex virus thymidine kinase gene and ganciclovir system(HSV TK/GCV) on humoral and cellular immunological functions in malignant glioma patients.Methods:Twelve patients of malignant glioma were treated with the HSV TK/GCV system. Peripheral blood and cerebral spinal fluid(CSF) were drawn before, during and afer treatment. Flow cytometry was used to analyze the T lymphocyte subclone of peripheral blood samples. Serum and CSF immunoglobins were quantified through turbidimetry. Results: CD4 +T lymphocyte was found elevated during the treatment; NK level fell during the treatment, but rose after treatment. After treatment, cytotoxic lymphocytes(CTL) level was significantly higher than before treatment. Serum IgM and CSF IgG, IgA and IgM levels were seen higher after treatment. Conclusion: HSV TK/GCV system can stimulate the humoral and cellular immunological functions in malignant glioma patients.

关 键 词：脑肿瘤 基因治疗 疱疹病毒 胞苷激酶 更昔洛韦 

分 类 号：R739.410.5[医药卫生—肿瘤] R730.51[医药卫生—临床医学]