大豆低聚糖对樱桃谷鸭肠道微生物群落结构的影响  

Effects of soybean oligosaccharide on intestinal microbial community structure in Cherry Valley ducks

作　　者：罗佳捷[1,2] 李丽立[1] 张彬[2] 王升平[1] 陈宇光[2] 陈登科[3] 

机构地区：[1]中国科学院亚热带农业生态研究所,长沙410125 [2]湖南农业大学经济动物研究所,长沙410128 [3]唐人神集团股份有限公司,湖南株洲412007

出　　处：《生态学杂志》2011年第6期1185-1189,共5页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30972118);湖南省自然科学基金项目(08JJ3080);长沙市重点科技攻关项目(K0802117-21)资助

摘　　要：为了探讨大豆低聚糖(SBOS)改善肉鸭肠道微生物群落结构的效果及机理,本试验运用末端限制性片段长度多态性(T-RFLP)方法研究了SBOS对樱桃谷鸭盲肠内容物中大肠杆菌、双歧杆菌和乳酸杆菌数量的影响。结果表明:与对照组相比,SBOS降低了盲肠内大肠杆菌的数量,其中第3、4组(添加300和500mg·kg-1SBOS)在第3、4周达到显著水平(P<0.05),第5组(添加700mg·kg-1SBOS)在第3周达到显著水平(P<0.05);SBOS对盲肠内双歧杆菌和乳酸杆菌有明显的增殖作用,其中在第3、4周,第4组2种菌的增殖达到显著水平(P<0.05);添加SBOS对于调节鸭肠道微生物群落效果与添加金霉素(100mg·kg-1)的效果差异不显著。SBOS能有效调节樱桃谷鸭肠道微生物群落结构,且呈剂量和时间依赖效应,本试验中,500mg·kg-1是最适添加量。In order to understand the effects of soybean oligosaccharide （SBOS） on the intestinal microbial community structure in Cherry Valley ducks,T-RFLP method was adopted to study the amounts of Escherichia coli,Bifidobacterium,and Lactobacillus in the duck caecum under effects of different concentration SBOS.Applying SBOS decreased the amount of E.coli in caecum.Compared with that of the control,the amount of E.coli in the 3rd and 4th week after applying 300 and 500 mg·kg-1 of SBOS and in the 3rd weed after applying 700 mg·kg-1 of SBOS had a significant decrease （P0.05）.Applying SBOS promoted the proliferation of Bifidobacterium and Lactobacillus,being significantly （P0.05） in the 3rd and 4th weeks after applying 500 mg·kg-1 of SBOS.No significant differences were observed between applying SBOS and aureomycin in the effect of regulating microbial community structure in duck intestine.Our results indicated that SBOS could effectively regulate the microbial community structure in Cherry Valley duck intestine,the effect was time- and dose-dependent,and the optimal additive dose was 500 mg·kg-1.

关 键 词：大豆低聚糖 微生物群落结构 樱桃谷鸭 末端限制性片段长度多态性 

分 类 号：S834[农业科学—畜牧学]