高产壳聚糖酶菌株的筛选及诱变育种  

Research on the Selection of High-yielding Chitosanase Strain and Its Mutagenesis Breeding

作　　者：王海波[1] 李轶群[1] 吴凯[1] 阎贺静[1] 

机构地区：[1]武汉生物工程学院,湖北武汉430415

出　　处：《安徽农业科学》2011年第14期8212-8214,共3页Journal of Anhui Agricultural Sciences

基　　金：武汉市教育局科研立项课题--新型壳聚糖酶的关键生产技术研究及其酶解生产壳寡糖(2010085);湖北省教育厅立项科技项目--用于壳寡糖酶解生产的关键酶制剂的研究与开发(B20114607)

摘　　要：[目的]研究利用紫外线和硫酸二乙酯(DES)诱变得到产壳聚糖酶活性较高的菌株。[方法]利用以壳聚糖为唯一碳源的选择性培养基,从自然界中筛选得到1株产壳聚糖酶活较高的菌株,对其进行鉴定,并且利用紫外线和DES诱变方法来提高该菌株的产酶能力。[结果]经初步鉴定,大致判定产壳聚糖酶的Y-4菌株为烟曲霉(Aspergillus fumigatus)。经紫外线和DES诱变处理分别得到2株壳聚糖酶活性明显提高的突变株,其酶活分别为8.92和8.12 U/ml,分别是出发菌株Y-4(3.00 U/ml)的3.0和2.7倍。2株突变株经连续传代10次后均具有较高的产酶稳定性。[结论]紫外线诱变较DES诱变效果好。[Objective] The research on the strain producing the chitosan with high activity,mutated with ultraviolet and diethyl sulfate（DES） was conducted.[Method] One strain of high-yielding chitosanase was isolated in the selective medium with the chitosan as sole carbon source and then was identified.Its ability in enzyme producing was improved through the treatment of UV and DES mutation.[Result] The preliminary identification indicated that the strain Y-4 producing the chitosanase might be one of Aspergillus fumigatus.The activity of two mutants,which was from the treatment of UV and DES,was 8.92 and 8.12 U/ml,respectively,which was 3.0 and 2.7 times of the strain Y-4（3.00 U/ml）,respectively.Two mutant strains had been stable in enzyme production after serial of 10 generations.[Conclusion] The effect of the ultraviolet radiation mutagenesis was better than that of DES.

关 键 词：壳聚糖酶 菌株 筛选 曲霉 诱变育种 

分 类 号：S188[农业科学—农业基础科学]